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Antagonism of Bacillus velezensis Isolate from Anaerobically Digested Dairy Slurry against Fusarium Wilt of Spinach

1
NARO Institute of Vegetable and Floriculture Science, Tsukuba 305-8519, Japan
2
Department of International Agricultural Development, Faculty of International Agriculture and Food Studies, Setagaya Campus, Tokyo University of Agriculture, Tokyo 156-8502, Japan
3
Department of Molecular Microbiology, Faculty of Life Sciences, Setagaya Campus, Tokyo University of Agriculture, Tokyo 156-8502, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Maryline Magnin-Robert
Agronomy 2022, 12(5), 1058; https://doi.org/10.3390/agronomy12051058
Received: 21 March 2022 / Revised: 13 April 2022 / Accepted: 27 April 2022 / Published: 28 April 2022
(This article belongs to the Special Issue Microbial Control of Crop Diseases: Limitations and Optimizations)
This study was designed to assess the suppressive effects of various anaerobically digested slurries (ADSs), and the microorganisms inhabiting them, against Fusarium wilt in spinach. We used five different ADSs from a range of source materials (dairy cow manure, sewage sludge, food garbage, pig manure, night soil sludge), combined in different proportions. All five raw ADSs suppressed the growth of Fusarium oxysporum f. sp. spinaciae (Fos) on agar plates using a co-culture test. In contrast, filtrate ADSs did not suppress the growth of Fos. In total, 32 bacterial strains were isolated from five ADSs, and eight isolates showed antagonistic activities against Fos. Based on 16S rDNA sequences, the strain AD-3 isolated from ADS from dairy cow manure belonged to Bacillus velezensis. Genome analysis revealed that AD-3 had two kinds of genes related to the production of the non-ribosomal lipopeptides, fengycin/plipastatin (pps genes), and surfactin (srf genes). In pot assays, inoculation of AD-3 (1.0 × 106 CFU·g−1 dry soil) into Fos-infected soil (1.0 × 105 bud-cells·g−1 dry soil) significantly reduced the severity of Fusarium wilt disease at 28 d after seedling. The percentage reductions in disease severity in two replicates were 64.3% and 44.3%, respectively. Thus, bacterial strain AD-3 could be applied to reduce Fusarium wilt in spinach. View Full-Text
Keywords: biological control; anaerobically digested dairy slurry; Bacillus velezensis biological control; anaerobically digested dairy slurry; Bacillus velezensis
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MDPI and ACS Style

Sugiyama, T.; Natsuaki, K.T.; Tanaka, N.; Shiwa, Y.; Irie, M. Antagonism of Bacillus velezensis Isolate from Anaerobically Digested Dairy Slurry against Fusarium Wilt of Spinach. Agronomy 2022, 12, 1058. https://doi.org/10.3390/agronomy12051058

AMA Style

Sugiyama T, Natsuaki KT, Tanaka N, Shiwa Y, Irie M. Antagonism of Bacillus velezensis Isolate from Anaerobically Digested Dairy Slurry against Fusarium Wilt of Spinach. Agronomy. 2022; 12(5):1058. https://doi.org/10.3390/agronomy12051058

Chicago/Turabian Style

Sugiyama, Tomomi, Keiko T. Natsuaki, Naoto Tanaka, Yuh Shiwa, and Mami Irie. 2022. "Antagonism of Bacillus velezensis Isolate from Anaerobically Digested Dairy Slurry against Fusarium Wilt of Spinach" Agronomy 12, no. 5: 1058. https://doi.org/10.3390/agronomy12051058

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