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Novel Poly-(Lactic-Co-Glycolic Acid) Targeted Nanoparticles Conjunct with Antibody for the Enhancement of Antibacterial Activity against Ralstonia solanacearum

1
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China
2
Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China
*
Author to whom correspondence should be addressed.
Academic Editor: Salvatore Davino
Agronomy 2021, 11(6), 1159; https://doi.org/10.3390/agronomy11061159
Received: 8 April 2021 / Revised: 30 May 2021 / Accepted: 1 June 2021 / Published: 5 June 2021
Due to the strong pathogenicity of Ralstonia solanacearum, a variety of strategies have been used to develop antibacterial agents; however, antibacterial drugs with targeted effects on R. solanacearum remain lacking. Herein, we present a nanoagent targeting R. solanacearum based on our previous research on poly-(lactic-co-glycolic acid) (PLGA) particles (PLGA-NPs) loaded with methyl caffeate and caffeic acid phenethyl ester. Antibodies that have specific effects on R. solanacearum, which were verified using immuno-PCR, were first used to prepare PLGA-targeted nanoparticles (PLGA-TNPs). The antibody coupling process was investigated in terms of antibody binding degree and antibacterial activity. The EC50 value of PLGA-TNPs was 0.021 mg/mL, which was significantly reduced by 92% in comparison to PLGA-NPs. PLGA-TNPs had a perforating effect on the cell membrane of R. solanacearum, but no effects on Escherichia coli according to the SEM results. In addition, a downregulation of the pathogenicity-related genes compared to PLGA-NP treatment was observed, and the expression of egl, phcA, phcB, pilT, polA-238, and pehC decreased by 78, 79, 87, 61, 58, and 41%, respectively. Therefore, PLGA-targeted nanoparticles not only enhance the activity against R. solanacearum, but also provide a new idea for controlling bacterial wilt. View Full-Text
Keywords: Ralstonia solanacearum; targeted drugs; nanoparticle; poly-(lactic-co-glycolic acid); caffeate Ralstonia solanacearum; targeted drugs; nanoparticle; poly-(lactic-co-glycolic acid); caffeate
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MDPI and ACS Style

Yang, X.-J.; Geng, L.-T.; Xu, X.-Y.; Shen, X.-Y.; Sheng, S.; Wu, F.-A.; Wang, J. Novel Poly-(Lactic-Co-Glycolic Acid) Targeted Nanoparticles Conjunct with Antibody for the Enhancement of Antibacterial Activity against Ralstonia solanacearum. Agronomy 2021, 11, 1159. https://doi.org/10.3390/agronomy11061159

AMA Style

Yang X-J, Geng L-T, Xu X-Y, Shen X-Y, Sheng S, Wu F-A, Wang J. Novel Poly-(Lactic-Co-Glycolic Acid) Targeted Nanoparticles Conjunct with Antibody for the Enhancement of Antibacterial Activity against Ralstonia solanacearum. Agronomy. 2021; 11(6):1159. https://doi.org/10.3390/agronomy11061159

Chicago/Turabian Style

Yang, Xue-Jun, Li-Tian Geng, Xiao-Yi Xu, Xiang-Yu Shen, Sheng Sheng, Fu-An Wu, and Jun Wang. 2021. "Novel Poly-(Lactic-Co-Glycolic Acid) Targeted Nanoparticles Conjunct with Antibody for the Enhancement of Antibacterial Activity against Ralstonia solanacearum" Agronomy 11, no. 6: 1159. https://doi.org/10.3390/agronomy11061159

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