Thiol-Ene Photo-Click Collagen-PEG Hydrogels: Impact of Water-Soluble Photoinitiators on Cell Viability, Gelation Kinetics and Rheological Properties
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Photo-Click Collagen-PEG Hydrogels
2.2.1. Extraction of Collagen Type I
2.2.2. Synthesis of Collagen-SH
2.2.3. Thiol-Ene Reaction
2.3. Chemical Characterisation
2.3.1. (2,4,6)-Trinitrobenzenesulfonic Acid (TNBS) Colorimetric Assay
2.3.2. Circular Dichroism
2.3.3. Ultraviolet/Visible (UV/Vis) Light Spectroscopy
2.4. In Vitro Cytotoxicity Assay
2.5. Rheology Studies
2.6. Network Architecture
2.6.1. Swelling Ratio and Gel Content
2.6.2. Morphology Study
2.7. Statistical Analysis
3. Results and Discussion
3.1. Synthesis and Evaluation of Collagen-SH
3.2. Photoinitiator Analysis
3.3. Thiol-Ene Gelation Kinetics via Rheological Studies
3.4. Network Architecture
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Photoinitiator (% w/v) | G’ (Pa) | τ (s) |
---|---|---|
I2959 (0.1) | 190 ± 22 | 1496 ± 43 |
I2959 (0.5) | 3029 ± 100 | 1683 ± 33 |
LAP (0.1) | 232 ± 39 | 279 ± 11 |
LAP (0.5) | 3360 ± 91 | 187 ± 6 |
Sampled ID | G’ (Pa) | G’’ (Pa) | τ (s) | Tanδ (×10−3) |
---|---|---|---|---|
CollPEG2 | 540 ± 23 | 5.1 ± 1.0 | 73 ± 3 | 5.3 ± 0.2 |
CollPEG2.5 | 1150 ± 46 | 5.8 ± 0.1 | 110 ± 6 | 6.3 ± 0.1 |
CollPEG3 | 2040 ± 50 | 8.6 ± 0.3 | 133 ± 6 | 5.7 ± 0.1 |
CollPEG3.5 | 3360 ± 91 | 11.1 ± 0.6 | 187 ± 6 | 5.0 ± 0.1 |
CollPEG4 | 4810 ± 41 | 13.2 ± 0.8 | 301 ± 13 | 3.5 ± 0.1 |
Sample ID | SR (wt %) | G (wt %) |
---|---|---|
CollPEG2 | 1530 ± 130 | 90 ± 1 |
CollPEG2.5 | 2130 ± 150 | 86 ± 3 |
CollPEG3 | 2340 ± 240 | 97 ± 1 |
CollPEG3.5 | 2640 ± 220 | 90 ± 2 |
CollPEG4 | 2840 ± 71 | 91 ± 1 |
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Holmes, R.; Yang, X.-B.; Dunne, A.; Florea, L.; Wood, D.; Tronci, G. Thiol-Ene Photo-Click Collagen-PEG Hydrogels: Impact of Water-Soluble Photoinitiators on Cell Viability, Gelation Kinetics and Rheological Properties. Polymers 2017, 9, 226. https://doi.org/10.3390/polym9060226
Holmes R, Yang X-B, Dunne A, Florea L, Wood D, Tronci G. Thiol-Ene Photo-Click Collagen-PEG Hydrogels: Impact of Water-Soluble Photoinitiators on Cell Viability, Gelation Kinetics and Rheological Properties. Polymers. 2017; 9(6):226. https://doi.org/10.3390/polym9060226
Chicago/Turabian StyleHolmes, Róisín, Xue-Bin Yang, Aishling Dunne, Larisa Florea, David Wood, and Giuseppe Tronci. 2017. "Thiol-Ene Photo-Click Collagen-PEG Hydrogels: Impact of Water-Soluble Photoinitiators on Cell Viability, Gelation Kinetics and Rheological Properties" Polymers 9, no. 6: 226. https://doi.org/10.3390/polym9060226