Next Article in Journal
Effect of Aging, Antioxidant, and Mono- and Divalent Ions at High Temperature on the Rheology of New Polyacrylamide-Based Co-Polymers
Next Article in Special Issue
Surfactant-Free RAFT Emulsion Polymerization of Styrene Using Thermoresponsive macroRAFT Agents: Towards Smart Well-Defined Block Copolymers with High Molecular Weights
Previous Article in Journal
Synthesis and Characterization of Organosoluble, Thermal Stable and Hydrophobic Polyimides Derived from 4-(4-(1-pyrrolidinyl)phenyl)-2,6-bis(4-(4-aminophenoxy)phenyl)pyridine
Previous Article in Special Issue
Combining Pickering Emulsion Polymerization with Molecular Imprinting to Prepare Polymer Microspheres for Selective Solid-Phase Extraction of Malachite Green
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Polymers 2017, 9(10), 485; https://doi.org/10.3390/polym9100485

Controlled Release of Lysozyme from Double-Walled Poly(Lactide-Co-Glycolide) (PLGA) Microspheres

1
Faculty of Pharmacy, International Islamic University Malaysia, Kuantan 25200, Malaysia
2
Department of Chemistry, University of Rajshahi, Rajshahi 6205, Bangladesh
3
Institute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, Kuala Nerus 21300, Terengganu, Malaysia
4
Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Nerus 21300, Terengganu, Malaysia
5
Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia
6
School of Ocean Engineering, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, Malaysia
7
Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Cyberjaya 63000, Malaysia
*
Author to whom correspondence should be addressed.
Received: 19 September 2017 / Revised: 28 September 2017 / Accepted: 28 September 2017 / Published: 3 October 2017
(This article belongs to the Special Issue Emulsion Polymerization)
Full-Text   |   PDF [3307 KB, uploaded 10 October 2017]   |  

Abstract

Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52% ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme. View Full-Text
Keywords: drug delivery; controlled release; double-walled microspheres; poly(lactide-co-glycolide); therapeutic proteins drug delivery; controlled release; double-walled microspheres; poly(lactide-co-glycolide); therapeutic proteins
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Ansary, R.H.; Rahman, M.M.; Mohamad, N.; Arrif, T.M.; Latif, A.Z.A.; Katas, H.; Nik, W.S.B.W.; Awang, M.B. Controlled Release of Lysozyme from Double-Walled Poly(Lactide-Co-Glycolide) (PLGA) Microspheres. Polymers 2017, 9, 485.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top