Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Silk Degumming with Different Neutral Proteases
2.2. Degumming Ratio
2.3. Morphological Characterization of Degummed Silk Fibers
2.4. Amino Acid Analysis
2.5. Thermal Analysis of Degummed Silk Fibers
2.6. Preparation of Regenerated SF Solution
2.7. Characterization of the Molecular Weight Distribution of Regenerated Silk Fibroin
2.8. Measurement for Shear Viscosity of Regenerated SF Solution
2.9. Statistical Analysis
3. Results
3.1. Degumming Ratio and Morphological Characterization of Degummed Silk Fibers
3.2. Amino Acid Analysis and Sericin Containing Ratio
3.3. Thermal Analysis
3.4. Characterization of the Molecular Weight Distribution of Regenerated Silk Fibroin
3.5. Viscosity of Regenerated SF Solution
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Enzyme | Concentration (g/L) | pH | Temperature (°C) | Each Time (min) |
---|---|---|---|---|
Subtilisin | 4 | 7 | 50 | 60 |
Trypsin | 4 | 6.8 | 38 | 60 |
Bromelain | 4 | 6.4 | 40 | 60 |
Papain | 2 | 6.6 | 55 | 60 |
Na2CO3 | 0.5 | / | 100 | 30 |
Amino Acids | Subtilisin | Trypsin | Bromelain | Papain | Na2CO3 | Raw Silk |
---|---|---|---|---|---|---|
Asp | 1.75 ± 0.04 | 1.76 ± 0.03 | 1.78 ± 0.01 | 1.73 ± 0.01 | 1.69 ± 0.01 | 3.94 ± 0.03 |
Thr | 0.98 ± 0.02 | 0.91 ± 0.07 | 0.97 ± 0.01 | 0.89 ± 0.14 | 0.93 ± 0.01 | 2.36 ± 0.04 |
Ser | 10.08 ± 0.18 | 11.91 ± 1.38 | 10.78 ± 0.15 | 10.92 ± 0.02 | 10.85 ± 0.08 | 14.67 ± 0.21 |
Glu | 1.86 ± 0.06 | 1.01 ± 1.17 | 1.93 ± 0.17 | 2.03 ± 0.18 | 1.57 ± 0.01 | 2.36 ± 0.21 |
Gly | 42.21 ± 0.08 | 41.33 ± 0.45 | 41.70 ± 0.76 | 41.78 ± 0.48 | 43.30 ± 0.52 | 37.89 ± 0.16 |
Ala | 31.41 ± 0.47 | 31.73 ± 0.32 | 31.84 ± 0.15 | 31.13 ± 0.07 | 31.12 ± 0.27 | 27.19 ± 0.10 |
Val | 2.29 ± 0.04 | 2.32 ± 0.10 | 2.36 ± 0.07 | 2.43 ± 0.23 | 2.22 ± 0.00 | 2.46 ± 0.03 |
Ile | 0.56 ± 0.03 | 0.55 ± 0.04 | 0.60 ± 0.03 | 0.6 ± 0.08 | 0.56 ± 0.01 | 0.58 ± 0.02 |
Leu | 0.54 ± 0.00 | 0.54 ± 0.05 | 0.54 ± 0.00 | 0.60 ± 0.08 | 0.51 ± 0.01 | 0.62 ± 0.04 |
Tyr | 5.04 ± 0.16 | 4.91 ± 0.20 | 4.92 ± 0.01 | 5.17 ± 0.02 | 5.04 ± 0.12 | 4.63 ± 0.08 |
Phe | 0.86 ± 0.06 | 0.80 ± 0.05 | 0.85 ± 0.06 | 0.88 ± 0.03 | 0.74 ± 0.04 | 0.71 ± 0.05 |
Lys | 0.34 ± 0.04 | 0.34 ± 0.01 | 0.34 ± 0.04 | 0.34 ± 0.03 | 0.27 ± 0.04 | 0.71 ± 0.02 |
His | 0.25 ± 0.03 | 0.24 ± 0.03 | 0.25 ± 0.03 | 0.25 ± 0.04 | 0.21 ± 0.01 | 0.36 ± 0.02 |
Arg | 0.46 ± 0.01 | 0.46 ± 0.03 | 0.46 ± 0.01 | 0.44 ± 0.01 | 0.45 ± 0.01 | 0.85 ± 0.05 |
Pro | 0.70 ± 0.16 | 0.64 ± 0.17 | 0.71 ± 0.13 | 0.79 ± 0.04 | 0.56 ± 0.01 | 0.67 ± 0.06 |
Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
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Liu, X.; Huang, Q.; Pan, P.; Fang, M.; Zhang, Y.; Yang, S.; Li, M.; Liu, Y. Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases. Polymers 2023, 15, 3383. https://doi.org/10.3390/polym15163383
Liu X, Huang Q, Pan P, Fang M, Zhang Y, Yang S, Li M, Liu Y. Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases. Polymers. 2023; 15(16):3383. https://doi.org/10.3390/polym15163383
Chicago/Turabian StyleLiu, Xueping, Qian Huang, Peng Pan, Mengqi Fang, Yadong Zhang, Shanlong Yang, Mingzhong Li, and Yu Liu. 2023. "Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases" Polymers 15, no. 16: 3383. https://doi.org/10.3390/polym15163383