Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Electrospinning
2.3. NDM Characterization
2.4. Encapsulation Efficiency (EE)
2.5. In Vitro TMP Release Profiles
2.6. Extraction of NDM Immersion Solution
2.7. Cell Culture
2.8. OGD Challenge
2.9. Cell Viability
2.10. Lactate Dehydrogenase (LDH) Release Assay
2.11. Cell Morphology
2.12. Immunofluorescence
2.13. Statistical Analysis
3. Results and Discussion
3.1. NDM Characterization
3.2. Encapsulation Efficiency and In Vitro TMP Release Profiles
3.3. PLGA/CS NDMs Inhibit the Excessive Proliferation of Fibroblasts
3.4. PLGA/CS NDMs Promote the Survival of OGD-Treated SH-SY5Y Cells
3.5. The Working Concentration of TMP with Neuroprotective Effect
3.6. PLGA/TMP/CS NDMs Promote the Survival of OGD-Treated SH-SY5Y Cells
3.7. PLGA/TMP/CS NDM Promote Nerve Repair
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, Z.; Wu, T.; Cui, Y.; Zhao, R.; Wan, Q.; Xu, R. Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells. Polymers 2022, 14, 1882. https://doi.org/10.3390/polym14091882
Zhao Z, Wu T, Cui Y, Zhao R, Wan Q, Xu R. Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells. Polymers. 2022; 14(9):1882. https://doi.org/10.3390/polym14091882
Chicago/Turabian StyleZhao, Zhiyuan, Tong Wu, Yu Cui, Rui Zhao, Qi Wan, and Rui Xu. 2022. "Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells" Polymers 14, no. 9: 1882. https://doi.org/10.3390/polym14091882