Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Micropatterned Surfaces
2.2. Characterization of Micropatterned Surfaces
2.3. Coating of Fibronectin and Immunological Staining of Coated Fibronectin
2.4. Cell Culture
2.5. TUNEL Assay
2.6. Staining of Actin Filaments
2.7. Analysis of DNA Synthesis Activity by BrdU Staining
2.8. Immunofluorescent Staining of P Glycoprotein
2.9. Statistical Analysis
3. Results and Discussions
3.1. Preparation and Characterization of Micropatterned Surfaces
3.2. Coating of Fibronectin and Cell Culture on Micropatterned Surfaces
3.3. Doxorubicin-Induced Apoptosis of Breast Cancer Cells on Micropatterned Surfaces
3.4. Cytoskeletal Organization of Breast Cancer Cells on Micropatterned Surfaces
3.5. DNA Synthesis Activity of Breast Cancer Cells on Micropatterned Surfaces
3.6. Staining of P-Glycoprotein of Breast Cancer Cells on Micropatterned Surfaces
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zheng, J.; Sun, R.; Chen, H.; Zeng, T.; Yoshitomi, T.; Kawazoe, N.; Yang, Y.; Chen, G. Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces. Polymers 2022, 14, 2761. https://doi.org/10.3390/polym14142761
Zheng J, Sun R, Chen H, Zeng T, Yoshitomi T, Kawazoe N, Yang Y, Chen G. Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces. Polymers. 2022; 14(14):2761. https://doi.org/10.3390/polym14142761
Chicago/Turabian StyleZheng, Jing, Rui Sun, Huajian Chen, Tianjiao Zeng, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, and Guoping Chen. 2022. "Morphological Dependence of Breast Cancer Cell Responses to Doxorubicin on Micropatterned Surfaces" Polymers 14, no. 14: 2761. https://doi.org/10.3390/polym14142761