Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characteristics of CSSA
2.2. Preparation and Characteristics of CSSA/siRNA
2.3. Cytotoxicity
2.4. Internalization of FAM-siRNA
2.5. Cell Survival Rate with X-ray Radiation
3. Materials and Methods
3.1. Materials
3.2. Cell Culture
3.3. Design and Synthesis of siRNA
- sense, 5′-GGAAUUCAAUGCUGAUGAUTT-3′;
- antisense, 5′-AUCAUCAGCAUUGAAUUCCTT-3′.
3.4. Synthesis of CSSA
3.5. Preparation of CSSA/siRNA Complex and Gel Retardation Assay
3.6. Characterization of CSSA and CSSA/siRNA
3.6.1. Size and Zeta Potential
3.6.2. Substitution Degree of Amino Groups
3.6.3. Critical Micelle Concentration
3.7. Cytotoxicity Assay
3.8. Internalization of CSSA Micelles
3.9. Determination of Cell Survival Rate with X-Ray Radiation
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Micelles | dn (nm) | PDI | Zeta Potential (mV) | SD (%) | CMC (μg/mL) |
---|---|---|---|---|---|
CSSA | 144.7 ± 3.2 | 0.212 ± 0.031 | 39.7 ± 0.8 | 15.70 ± 1.02 | 75.02 ± 1.27 |
CSSA/siRNA | 168.1 ± 2.9 | 0.136 ± 0.025 | 20.3 ± 0.5 | - | - |
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Miao, J.; Zhang, L.; Gao, P.; Zhao, H.; Xie, X.; Wang, J. Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma. Polymers 2021, 13, 2929. https://doi.org/10.3390/polym13172929
Miao J, Zhang L, Gao P, Zhao H, Xie X, Wang J. Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma. Polymers. 2021; 13(17):2929. https://doi.org/10.3390/polym13172929
Chicago/Turabian StyleMiao, Jing, Liwen Zhang, Peng Gao, Huawei Zhao, Xianji Xie, and Junyan Wang. 2021. "Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma" Polymers 13, no. 17: 2929. https://doi.org/10.3390/polym13172929