Applied Rheology as Tool for the Assessment of Chitosan Hydrogels for Regenerative Medicine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Hydrogels Production
2.3. Hydrogels Characterization
2.3.1. Rheological Characterization
2.3.2. Microstructural Characterization
2.4. Statistical Analyses
3. Results and Discussion
3.1. Study of the pH Change and Agitation Time
3.2. Study of the Gelation Temperature
3.3. Study of Chitosan Concentration
3.4. Microstructure Evaluation
3.5. Temperature Study
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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System | Chitosan Concentration (wt.%) | Agitation Time (h) | pH Change | Final Temperature (°C) |
---|---|---|---|---|
1 | 1.5 | 1 | Yes | 50 |
2 | 1.5 | 1 | No | 50 |
3 | 1.5 | 2 | Yes | 50 |
4 | 1.5 | 2 | No | 50 |
5 | 1.5 | 1 | Yes | 20 |
6 | 1.5 | 1 | Yes | 4 |
7 | 1.0 | 1 | Yes | 4 |
8 | 2.0 | 1 | Yes | 4 |
Systems | Critical Strain (%) | G′1 (Pa) | tan(δ)1 (-) | η*1 (Pa·s) | |
---|---|---|---|---|---|
Agitation Time | pH Change | ||||
1 h | No | 1.01 ± 0.05 I | 3.47 ± 0.42 a | 2.04 ± 0.03 A | 0.99 ± 0.26 α |
Yes | 0.48 ± 0.08 II | 1117 ± 12 b | 0.11 ± 0.01 B | 184 ± 29 β | |
2 h | No | 0.42 ± 0.08 II | 0.38 ± 0.07 c | 2.93 ± 0.05 C | 0.57 ± 0.17 α |
Yes | 0.48 ± 0.11 II | 1456 ± 22 d | 0.04 ± 0.01 D | 191 ± 43 β |
Gelation Temperature | Critical Strain (%) | G′1 (Pa) | tan(δ)1 (-) | η*1 (Pa·s) |
---|---|---|---|---|
4 °C | 1.01 ± 0.11 I | 1122 ± 15 a | 0.05 ± 0.02 A | 142 ± 17 α |
20 °C | 1.01 ± 0.05 I | 720 ± 12 b | 0.06 ± 0.03 A | 91 ± 16 β |
50 °C | 0.48 ± 0.08 II | 1117 ± 12 a | 0.11 ± 0.01 B | 184 ± 29 γ |
Biopolymer Concentration | Critical Strain (%) | G′1 (Pa) | tan(δ)1 (-) | η*1 (Pa·s) |
---|---|---|---|---|
1.0 wt.% | 0.48 ± 0.08 I | 130 ± 12 a | 0.08 ± 0.01 A | 21 ± 6 α |
1.5 wt.% | 1.01 ± 0.11 II | 1122 ± 15 b | 0.05 ± 0.02 A | 142 ± 17 β |
2.0 wt.% | 1.01 ± 0.11 II | 1049 ± 97 b | 0.06 ± 0.04 A | 201 ± 21 γ |
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Sánchez-Cid, P.; Jiménez-Rosado, M.; Alonso-González, M.; Romero, A.; Perez-Puyana, V. Applied Rheology as Tool for the Assessment of Chitosan Hydrogels for Regenerative Medicine. Polymers 2021, 13, 2189. https://doi.org/10.3390/polym13132189
Sánchez-Cid P, Jiménez-Rosado M, Alonso-González M, Romero A, Perez-Puyana V. Applied Rheology as Tool for the Assessment of Chitosan Hydrogels for Regenerative Medicine. Polymers. 2021; 13(13):2189. https://doi.org/10.3390/polym13132189
Chicago/Turabian StyleSánchez-Cid, Pablo, Mercedes Jiménez-Rosado, María Alonso-González, Alberto Romero, and Victor Perez-Puyana. 2021. "Applied Rheology as Tool for the Assessment of Chitosan Hydrogels for Regenerative Medicine" Polymers 13, no. 13: 2189. https://doi.org/10.3390/polym13132189