Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Gelatin-Methacryloyl
2.2. Determination of GelMa Degree of Substitution
2.3. Preparation of GelMa Hydrogels
2.4. Hydrogel Swelling Analysis
2.5. Rheological Measurement
2.6. Mechanical Testing
2.7. Enzymatic Degradation of Hydrogels
2.8. Culture of Fibroblasts in Hydrogel
2.9. Live/Dead Staining and Cell Viability
2.10. Cytoskeleton Staining
2.11. Western Blot Analysis
2.12. Immunocytochemistry
2.13. Scaffold Fabrication
2.14. Statistical Analyses
3. Results and Discussion
3.1. Determination of Degree of Methacrylation
3.2. Sol-gel Transition Temperature of Hydrogel
3.3. Swelling Characteristics
3.4. Mechanical Properties
3.5. Degradation
3.6. In Vitro hDF Culture
3.7. Cell Remodeling
3.8. hDF-laden Scaffold Fabrication
3.9. Cell Proliferation
3.10. Protein Expression of hDF-laden Scaffold
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Shie, M.-Y.; Lee, J.-J.; Ho, C.-C.; Yen, S.-Y.; Ng, H.Y.; Chen, Y.-W. Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior. Polymers 2020, 12, 1930. https://doi.org/10.3390/polym12091930
Shie M-Y, Lee J-J, Ho C-C, Yen S-Y, Ng HY, Chen Y-W. Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior. Polymers. 2020; 12(9):1930. https://doi.org/10.3390/polym12091930
Chicago/Turabian StyleShie, Ming-You, Jian-Jr Lee, Chia-Che Ho, Ssu-Yin Yen, Hooi Yee Ng, and Yi-Wen Chen. 2020. "Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior" Polymers 12, no. 9: 1930. https://doi.org/10.3390/polym12091930