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Article

Water-Soluble and Cytocompatible Phospholipid Polymers for Molecular Complexation to Enhance Biomolecule Transportation to Cells In Vitro

1
Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
2
Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba-Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(8), 1762; https://doi.org/10.3390/polym12081762
Received: 13 July 2020 / Revised: 28 July 2020 / Accepted: 4 August 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Polymer Micelles II)
Water-soluble and cytocompatible polymers were investigated to enhance a transporting efficiency of biomolecules into cells in vitro. The polymers composed of a 2-methacryloyloxyethyl phosphorylcholine (MPC) unit, a hydrophobic monomer unit, and a cationic monomer unit bearing an amino group were synthesized for complexation with model biomolecules, siRNA. The cationic MPC polymer was shown to interact with both siRNA and the cell membrane and was successively transported siRNA into cells. When introducing 20–50 mol% hydrophobic units into the cationic MPC polymer, transport of siRNA into cells. The MPC units (10–20 mol%) in the cationic MPC polymer were able to impart cytocompatibility, while maintaining interaction with siRNA and the cell membrane. The level of gene suppression of the siRNA/MPC polymer complex was evaluated in vitro and it was as the same level as that of a conventional siRNA transfection reagent, whereas its cytotoxicity was significantly lower. We concluded that these cytocompatible MPC polymers may be promising complexation reagent for introducing biomolecules into cells, with the potential to contribute to future fields of biotechnology, such as in vitro evaluation of gene functionality, and the production of engineered cells with biological functions. View Full-Text
Keywords: 2-methacryloyloxyethyl phosphorylcholine polymer; amphiphilic nature; cationic group; polymer aggregate; endocytosis 2-methacryloyloxyethyl phosphorylcholine polymer; amphiphilic nature; cationic group; polymer aggregate; endocytosis
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MDPI and ACS Style

Ishihara, K.; Hachiya, S.; Inoue, Y.; Fukazawa, K.; Konno, T. Water-Soluble and Cytocompatible Phospholipid Polymers for Molecular Complexation to Enhance Biomolecule Transportation to Cells In Vitro. Polymers 2020, 12, 1762. https://doi.org/10.3390/polym12081762

AMA Style

Ishihara K, Hachiya S, Inoue Y, Fukazawa K, Konno T. Water-Soluble and Cytocompatible Phospholipid Polymers for Molecular Complexation to Enhance Biomolecule Transportation to Cells In Vitro. Polymers. 2020; 12(8):1762. https://doi.org/10.3390/polym12081762

Chicago/Turabian Style

Ishihara, Kazuhiko, Shohei Hachiya, Yuuki Inoue, Kyoko Fukazawa, and Tomohiro Konno. 2020. "Water-Soluble and Cytocompatible Phospholipid Polymers for Molecular Complexation to Enhance Biomolecule Transportation to Cells In Vitro" Polymers 12, no. 8: 1762. https://doi.org/10.3390/polym12081762

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