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Article

Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks

1
Regenerative Medicine and Cellular Therapies Group, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK
2
UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, INSERM, ONIRIS, F-44042 Nantes, France
3
UFR Odontologie, Université de Nantes, 44042 Nantes, France
4
Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, UK
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(6), 1260; https://doi.org/10.3390/polym12061260
Received: 21 April 2020 / Revised: 28 May 2020 / Accepted: 28 May 2020 / Published: 30 May 2020
(This article belongs to the Special Issue 3D and 4D Printing of (Bio)Materials)
The survival and function of thick tissue engineered implanted constructs depends on pre-existing, embedded, functional, vascular-like structures that are able to integrate with the host vasculature. Bioprinting was employed to build perfusable vascular-like networks within thick constructs. However, the improvement of oxygen transportation facilitated by these vascular-like networks was directly quantified. Using an optical fiber oxygen sensor, we measured the oxygen content at different positions within 3D bioprinted constructs with and without perfusable microchannel networks. Perfusion was found to play an essential role in maintaining relatively high oxygen content in cell-laden constructs and, consequently, high cell viability. The concentration of oxygen changes following switching on and off the perfusion. Oxygen concentration depletes quickly after pausing perfusion but recovers rapidly after resuming the perfusion. The quantification of oxygen levels within cell-laden hydrogel constructs could provide insight into channel network design and cellular responses. View Full-Text
Keywords: 3D bioprinting; microfluidics; microchannels; oxygen; hydrogel; silated-HPMC 3D bioprinting; microfluidics; microchannels; oxygen; hydrogel; silated-HPMC
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    Doi: 10.5281/zenodo.3759474
    Description: Perfusion of the 3D printed construct after removal of the sacrificial ink (8x8x6mm)..
MDPI and ACS Style

Figueiredo, L.; Le Visage, C.; Weiss, P.; Yang, J. Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks. Polymers 2020, 12, 1260. https://doi.org/10.3390/polym12061260

AMA Style

Figueiredo L, Le Visage C, Weiss P, Yang J. Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks. Polymers. 2020; 12(6):1260. https://doi.org/10.3390/polym12061260

Chicago/Turabian Style

Figueiredo, Lara, Catherine Le Visage, Pierre Weiss, and Jing Yang. 2020. "Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks" Polymers 12, no. 6: 1260. https://doi.org/10.3390/polym12061260

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