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Article

Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

1
Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic
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Office of Research and Management, c/o Institute of Chemistry, NAWI Graz, University of Graz, Heinrichstrasse 28/VI, 8010 Graz, Austria
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ARENA Arbeitsgemeinschaft für Ressourcenschonende & Nachhaltige Technologien, Inffeldgasse 21b, 8010 Graz, Austria
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Biology Centre, The Czech Academy of Sciences, v.v.i., Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
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Faculty of Science, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
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Institute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Kralovopolska 147, 612 64 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(6), 1235; https://doi.org/10.3390/polym12061235
Received: 16 April 2020 / Revised: 15 May 2020 / Accepted: 26 May 2020 / Published: 29 May 2020
(This article belongs to the Special Issue Sustainable Polymers from Biomass)
Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolate Aneurinibacillus sp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 °C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated that Aneurinibacillus sp. H1 is a very promising candidate for production of PHA with tailored material properties. View Full-Text
Keywords: polyhydroxyalkanoates; thermophiles; Aneurinibacillus sp.; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB) polyhydroxyalkanoates; thermophiles; Aneurinibacillus sp.; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)
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MDPI and ACS Style

Pernicova, I.; Novackova, I.; Sedlacek, P.; Kourilova, X.; Kalina, M.; Kovalcik, A.; Koller, M.; Nebesarova, J.; Krzyzanek, V.; Hrubanova, K.; Masilko, J.; Slaninova, E.; Obruca, S. Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium. Polymers 2020, 12, 1235. https://doi.org/10.3390/polym12061235

AMA Style

Pernicova I, Novackova I, Sedlacek P, Kourilova X, Kalina M, Kovalcik A, Koller M, Nebesarova J, Krzyzanek V, Hrubanova K, Masilko J, Slaninova E, Obruca S. Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium. Polymers. 2020; 12(6):1235. https://doi.org/10.3390/polym12061235

Chicago/Turabian Style

Pernicova, Iva, Ivana Novackova, Petr Sedlacek, Xenie Kourilova, Michal Kalina, Adriana Kovalcik, Martin Koller, Jana Nebesarova, Vladislav Krzyzanek, Kamila Hrubanova, Jiri Masilko, Eva Slaninova, and Stanislav Obruca. 2020. "Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium" Polymers 12, no. 6: 1235. https://doi.org/10.3390/polym12061235

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