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Open AccessArticle

Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules

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Department of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, Colombia
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Laboratorio de Nanotecnología Ambiental, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Monterrey 64849, Mexico
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Department of Biomedical Engineering, Universidad de Los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, Colombia
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(5), 1158; https://doi.org/10.3390/polym12051158
Received: 30 March 2020 / Revised: 20 April 2020 / Accepted: 27 April 2020 / Published: 19 May 2020
The development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation of the fungal laccase from Pycnoporus sanguineus CS43 in alginate microcapsules. Multiphysics simulations were used to overview the fluid behavior within the device and estimate the resulting capsule size. Polymethylmethacrylate (PMMA) sheets were used for final microsystem manufacture. Different flow rates of the continuous (Qc) and discrete (Qd) phases in the ranges of 83–293 mL/h and 1–5 mL/h, respectively, were evaluated for microcapsule fabrication. Universal Serial Bus (USB) microscope and image analysis was used to measure the final particle size. Laccase encapsulation was evaluated using spectrophotometry and with the aid of fluorescent dyes and confocal microscopy. Results showed microcapsule size was in the range of 203.13–716.00 μm and Qc was found as the dominant parameter to control capsule size. There was an effective enzyme encapsulation of 65.94% with respect to the initial laccase solution. View Full-Text
Keywords: microfluidics; microcapsules; alginate; laccase microfluidics; microcapsules; alginate; laccase
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MDPI and ACS Style

Campaña, A.L.; Sotelo, D.C.; Oliva, H.A.; Aranguren, A.; Ornelas-Soto, N.; Cruz, J.C.; Osma, J.F. Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules. Polymers 2020, 12, 1158.

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