Next Article in Journal
Qualification of New Methods for Measuring In Situ Rheology of Non-Newtonian Fluids in Porous Media
Previous Article in Journal
High-Conductivity, Flexible and Transparent PEDOT:PSS Electrodes for High Performance Semi-Transparent Supercapacitors
Open AccessArticle

ECM Mimetic Electrospun Porous Poly (L-lactic acid) (PLLA) Scaffolds as Potential Substrates for Cardiac Tissue Engineering

1
Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
2
Bio-Nano Electronics Research Centre, Toyo University, Kawagoe, Saitama 350-8585, Japan
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(2), 451; https://doi.org/10.3390/polym12020451 (registering DOI)
Received: 7 January 2020 / Revised: 10 February 2020 / Accepted: 11 February 2020 / Published: 14 February 2020
(This article belongs to the Section Polymer Applications)
Cardiac tissue engineering (CTE) aims to generate potential scaffolds to mimic extracellular matrix (ECM) for recreating the injured myocardium. Highly porous scaffolds with properties that aid cell adhesion, migration and proliferation are critical in CTE. In this study, electrospun porous poly (l-lactic acid) (PLLA) porous scaffolds were fabricated and modified with different ECM derived proteins such as collagen, gelatin, fibronectin and poly-L-lysine. Subsequently, adult human cardiac fibroblasts (AHCF) were cultured on the protein modified and unmodified fibers to study the cell behavior and guidance. Further, the cytotoxicity and reactive oxygen species (ROS) assessments of the respective fibers were performed to determine their biocompatibility. Excellent cell adhesion and proliferation of the cardiac fibroblasts was observed on the PLLA porous fibers regardless of the surface modifications. The metabolic rate of cells was on par with the conventional cell culture ware while the proliferation rate surpassed the latter by nearly two-folds. Proteome profiling revealed that apart from being an anchorage platform for cells, the surface topography has modulated significant expression of the cellular proteome with many crucial proteins responsible for cardiac fibroblast growth and proliferation.
Keywords: cardiac tissue engineering; electrospinning; porous polymeric fibers; cell scaffold; extracellular matrix cardiac tissue engineering; electrospinning; porous polymeric fibers; cell scaffold; extracellular matrix
Show Figures

Graphical abstract

MDPI and ACS Style

Muniyandi, P.; Palaninathan, V.; Veeranarayanan, S.; Ukai, T.; Maekawa, T.; Hanajiri, T.; Mohamed, M.S. ECM Mimetic Electrospun Porous Poly (L-lactic acid) (PLLA) Scaffolds as Potential Substrates for Cardiac Tissue Engineering. Polymers 2020, 12, 451.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop