Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Porosity and Density
- —initial volume of isopropanol [cm3],
- —total volume of isopropanol and isopropanol impregnated scaffold [cm3],
- —isopropanol volume after sample removing [cm3],
- —sample weight [mg].
2.3. Microstructure of the Scaffolds
2.4. Swelling Behaviour and Moisture Content
- —weight of the material after immersion in PBS [mg],
- —weight of the material before immersion [mg].
2.5. Biological Tests
- —fluorescence of the samples,
- —fluorescence of EMEM with 5% AlamarBlue reagent but without cells (0% reduction of resazurin),
- —fluorescence of EMEM with 5% AlamarBlue reagent autoclaved for 15 min at 121 °C (100% reduction of resazurin).
3. Results and Discussion
3.1. Porosity and Density
3.2. Microstructure of the Scaffolds
3.3. Swelling Behaviour and Moisture Content
3.4. Biological Tests with Osteoblast-Like MG-63 Cells
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Porosity [%] | Density [mg/cm3] | ||
---|---|---|---|---|
without DAS | with DAS | without DAS | with DAS | |
SF | 95 ± 1.7 | 86 ± 4.0 | 33.0 ± 0.4 | 34.6 ± 2.4 |
Coll | 88 ± 0.5 | 89 ± 4.0 | 16.9 ± 2.2 | 16.0 ± 1.4 |
SF/Coll | 97 ± 1.0 | 91 ± 0.9 | 14.8 ± 2.1 | 17.7 ± 0.4 |
SF/Coll/CTS | 96 ± 1.9 | 93 ± 1.9 | 17.0 ± 1.5 | 19.0 ± 0.7 |
Sample | Swelling Ratio [%] | Moisture Content in 100 g of Dry Sample [g] | ||
---|---|---|---|---|
without DAS | with DAS | without DAS | with DAS | |
SF | 1511 ± 147 | 1792 ± 93 | 7.09 ± 1.03 | 12.09 ± 0.50 |
Coll | Nd | 1084 ± 48 | 14.17 ± 1.36 | 13.10 ± 0.79 |
SF/Coll | 1891 ± 236 | 2091 ± 136 | 19.27 ± 0.92 | 17.33 ± 0.30 |
SF/Coll/CTS | 1678 ± 19 | 2102 ± 9 | 22.43 ± 1.32 | 19.60 ± 1.35 |
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Grabska-Zielińska, S.; Sionkowska, A.; Reczyńska, K.; Pamuła, E. Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch. Polymers 2020, 12, 372. https://doi.org/10.3390/polym12020372
Grabska-Zielińska S, Sionkowska A, Reczyńska K, Pamuła E. Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch. Polymers. 2020; 12(2):372. https://doi.org/10.3390/polym12020372
Chicago/Turabian StyleGrabska-Zielińska, Sylwia, Alina Sionkowska, Katarzyna Reczyńska, and Elżbieta Pamuła. 2020. "Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch" Polymers 12, no. 2: 372. https://doi.org/10.3390/polym12020372