Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Yoshida, Kentaro; Kashimura, Yu; Kamijo, Toshio; Ono, Tetsuya; Dairaku, Takenori; Sato, Takaya; Kashiwagi, Yoshitomo; Sato, Katsuhiko

doi:10.3390/polym12020319

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Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

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School of Pharmaceutical Sciences, Ohu University 31-1 Misumido, Tomita-machi, Koriyama, Fukushima 963-8611, Japan

Department of Creative Engineering, National Institute of Technology, Tsuruoka College, 104 Sawada, Inooka, Tsuruoka 997-8511, Japan

Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan

Author to whom correspondence should be addressed.

Polymers 2020, 12(2), 319; https://doi.org/10.3390/polym12020319

Received: 8 January 2020 / Revised: 30 January 2020 / Accepted: 3 February 2020 / Published: 4 February 2020

(This article belongs to the Special Issue Biomedical Polymer Materials II)

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Abstract

Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)₅ film was then partially decomposed by hydrogen peroxide (H₂O₂). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H₂O₂, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)₅ film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)₅ films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM. View Full-Text

Keywords: hydrogen peroxide response; layer-by-layer; multilayer thin film; glucose sensitive; stimuli-sensitive

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MDPI and ACS Style

Yoshida, K.; Kashimura, Y.; Kamijo, T.; Ono, T.; Dairaku, T.; Sato, T.; Kashiwagi, Y.; Sato, K. Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase. Polymers 2020, 12, 319. https://doi.org/10.3390/polym12020319

AMA Style

Yoshida K, Kashimura Y, Kamijo T, Ono T, Dairaku T, Sato T, Kashiwagi Y, Sato K. Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase. Polymers. 2020; 12(2):319. https://doi.org/10.3390/polym12020319

Chicago/Turabian Style

Yoshida, Kentaro, Yu Kashimura, Toshio Kamijo, Tetsuya Ono, Takenori Dairaku, Takaya Sato, Yoshitomo Kashiwagi, and Katsuhiko Sato. 2020. "Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase" Polymers 12, no. 2: 319. https://doi.org/10.3390/polym12020319

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Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

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