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Open AccessArticle

Enhanced Protein Immobilization on Polymers—A Plasma Surface Activation Study

Laboratory for Sensors, Department of Microsystems Engineering, University of Freiburg, 79110 Freiburg, Germany
Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, 79110 Freiburg, Germany
Research Center of Microtechnology, Vorarlberg University of Applied Sciences, Dornbirn 6850, Austria
Freiburg Materials Research Center, University of Freiburg, 79104 Freiburg, Germany
Author to whom correspondence should be addressed.
Polymers 2020, 12(1), 104;
Received: 19 November 2019 / Revised: 20 December 2019 / Accepted: 25 December 2019 / Published: 4 January 2020
(This article belongs to the Section Polymer Processing and Performance)
Over the last years, polymers have gained great attention as substrate material, because of the possibility to produce low-cost sensors in a high-throughput manner or for rapid prototyping and the wide variety of polymeric materials available with different features (like transparency, flexibility, stretchability, etc.). For almost all biosensing applications, the interaction between biomolecules (for example, antibodies, proteins or enzymes) and the employed substrate surface is highly important. In order to realize an effective biomolecule immobilization on polymers, different surface activation techniques, including chemical and physical methods, exist. Among them, plasma treatment offers an easy, fast and effective activation of the surfaces by micro/nanotexturing and generating functional groups (including carboxylic acids, amines, esters, aldehydes or hydroxyl groups). Hence, here we present a systematic and comprehensive plasma activation study of various polymeric surfaces by optimizing different parameters, including power, time, substrate temperature and gas composition. Thereby, the highest immobilization efficiency along with a homogenous biomolecule distribution is achieved with a 5-min plasma treatment under a gas composition of 50% oxygen and nitrogen, at a power of 1000 W and a substrate temperature of 80 °C. These results are also confirmed by different surface characterization methods, including SEM, XPS and contact angle measurements. View Full-Text
Keywords: surface activation; oxygen plasma; protein immobilization; biosensors surface activation; oxygen plasma; protein immobilization; biosensors
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MDPI and ACS Style

Wieland, F.; Bruch, R.; Bergmann, M.; Partel, S.; Urban, G.A.; Dincer, C. Enhanced Protein Immobilization on Polymers—A Plasma Surface Activation Study. Polymers 2020, 12, 104.

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