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Highly Selective Fluorescence Sensing and Imaging of ATP Using a Boronic Acid Groups-Bearing Polythiophene Derivate

1
School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China
2
Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, Tianjin University of Technology, Tianjin 300384, China
3
Tianjin Key Laboratory for Photoelectric Materials and Devices, Tianjin University of Technology, Tianjin 300384, China
*
Authors to whom correspondence should be addressed.
Polymers 2019, 11(7), 1139; https://doi.org/10.3390/polym11071139
Received: 8 June 2019 / Revised: 27 June 2019 / Accepted: 1 July 2019 / Published: 3 July 2019
(This article belongs to the Special Issue Fluorescent polymers for sensing and imaging)
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Abstract

A boronic acid groups-bearing polythiophene derivate (L) was designed and synthesized for highly sensitive fluorescence detection of ATP based on a multisite-binding coupled with analyte-induced aggregation strategy. L has a polythiophene backbone as fluorophores and two functional side groups, i.e., quaternary ammonium group and boronic acid group, as multibinding sites for ATP. When various structural analogues such as ADP, AMP, and various inorganic phosphates were added into the aqueous solution of L, only ATP caused a remarkable fluorescence quenching of about 60-fold accompanied by obvious color changes of solution from yellow to purple. The detection limit is estimated to be 2 nM based on 3σ/slope. With the advantage of good water solubility, low toxicity, and highly selective response to ATP, L was successfully utilized as a probe to real-time assay activity of adenylate kinase (ADK) and map fluorescent imaging of ATP in living cells. View Full-Text
Keywords: ATP detection; fluorescent probe; polythiophene; multisite binding; analyte-induced aggregation ATP detection; fluorescent probe; polythiophene; multisite binding; analyte-induced aggregation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liu, L.; Zhao, L.; Cheng, D.; Yao, X.; Lu, Y. Highly Selective Fluorescence Sensing and Imaging of ATP Using a Boronic Acid Groups-Bearing Polythiophene Derivate. Polymers 2019, 11, 1139.

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