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Open AccessArticle

Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer

1
School of Materials Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
2
Shandong Provincial Key Laboratory of Processing and Testing Technology of Glass and Functional Ceramics; Key Laboratory of Amorphous and Polycrystalline Materials; Qilu University of Technology, Jinan 250353, China
*
Author to whom correspondence should be addressed.
The authors (Y.-l.Z. and Q.-b.W.) contributed equally to this work.
Polymers 2019, 11(10), 1643; https://doi.org/10.3390/polym11101643
Received: 31 August 2019 / Revised: 5 October 2019 / Accepted: 5 October 2019 / Published: 10 October 2019
(This article belongs to the Special Issue Surface Modification and Functionalization of Polymers)
The calcium ion (Ca2+) isa highly versatile intracellular signal messenger regulating many different cellular functions. It is important to design probes with good fluorescence and two-photon (TP) active cross-sections (Φδ) to explore the concentration distribution of Ca2+. In this manuscript, a novel TP fluorescence calcium probe (BAPTAVP) with positive charges, based on the classical Ca2+ indicator of BAPTA (1,2-bis(2-aminophenoxy)-ethane-N,N,N’,N’-tetra acetic acid), and a conjugated polymer (PCBMB) with negative charges were designed and synthesized. The results from transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), and the zeta potential (ZP) showed that nanoparticles were obtained by the self-assembly of PCBMB and BAPTAVP. Moreover, the fluorescence properties of BAPTAVP were effectively improved by fluorescence resonance energy transfer (FRET) with PCBMB and attenuating the intramolecular charge transfer (ICT) after the addition of Ca2+. The quantum yield and Φδ of PCBMB-BAPTAVP increased by about four and six times in comparison to those of BAPTAVP, respectively. The TP fluorescence imaging experiments indicated that the PCBMB-BAPTAVP system could effectively detect Ca2+ in living cells with high sensitivity. View Full-Text
Keywords: fluorescence probe; Ca2+; conjugated polymer; self-assembly; two-photon fluorescence imaging fluorescence probe; Ca2+; conjugated polymer; self-assembly; two-photon fluorescence imaging
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MDPI and ACS Style

Zhai, Y.-L.; Wang, Q.-B.; Yu, H.; Ji, X.-Y.; Zhang, X. Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer. Polymers 2019, 11, 1643.

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