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Polymers 2019, 11(1), 115; https://doi.org/10.3390/polym11010115

Glucose Oxidase Immobilized on a Functional Polymer Modified Glassy Carbon Electrode and Its Molecule Recognition of Glucose

1
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
2
Institute of Biochemistry and Biophysics, University of Tehran, Enquelab Avenue, Tehran 1417614418, Iran
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 20 November 2018 / Revised: 7 January 2019 / Accepted: 7 January 2019 / Published: 11 January 2019
(This article belongs to the Special Issue Bioactive and Biofunctional Conductive Polymers)
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Abstract

In the present study, a glucose oxidase (GluOx) direct electron transfer was realized on an aminated polyethylene glycol (mPEG), carboxylic acid functionalized multi-walled carbon nanotubes (fMWCNTs), and ionic liquid (IL) composite functional polymer modified glassy carbon electrode (GCE). The amino groups in PEG, carboxyl groups in multi-walled carbon nanotubes, and IL may have a better synergistic effect, thus more effectively adjust the hydrophobicity, stability, conductivity, and biocompatibility of the composite functional polymer film. The composite polymer membranes were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectrophotometer, fluorescence spectroscopy, electrochemical impedance spectroscopy (EIS), and transmission electron microscopy (TEM), respectively. In 50 mM, pH 7.0 phosphate buffer solution, the formal potential and heterogeneous electron transfer constant (ks) of GluOx on the composite functional polymer modified GCE were −0.27 V and 6.5 s−1, respectively. The modified electrode could recognize and detect glucose linearly in the range of 20 to 950 μM with a detection limit of 0.2 μM. The apparent Michaelis-Menten constant (Kmapp) of the modified electrode was 143 μM. The IL/mPEG-fMWCNTs functional polymer could preserve the conformational structure and catalytic activity of GluOx and lead to high sensitivity, stability, and selectivity of the biosensors for glucose recognition and detection. View Full-Text
Keywords: glucose oxidase; direct electrochemistry; functional polymer; aminated polyethylene glycol glucose oxidase; direct electrochemistry; functional polymer; aminated polyethylene glycol
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ning, Y.-N.; Xiao, B.-L.; Niu, N.-N.; Moosavi-Movahedi, A.A.; Hong, J. Glucose Oxidase Immobilized on a Functional Polymer Modified Glassy Carbon Electrode and Its Molecule Recognition of Glucose. Polymers 2019, 11, 115.

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