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Polymers 2018, 10(2), 151; https://doi.org/10.3390/polym10020151

Sortase-Mediated Ligation of Purely Artificial Building Blocks

1
Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam-Golm, Germany
2
Lehrstuhl für Polymermaterialien und Polymertechnologie, University of Potsdam, 14476 Potsdam-Golm, Germany
3
DWI—Leibniz Institute for Interactive Materials e.V., Forckenbeckstr. 50, 52056 Aachen, Germany
4
Institute of Biotechnology, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany
Current address: Center of Molecular Biology “Severo Ochoa”, Universidad Autónoma de Madrid, Nicolás Cabrera 1, 28049 Madrid, Spain.
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 December 2017 / Revised: 19 January 2018 / Accepted: 2 February 2018 / Published: 6 February 2018
(This article belongs to the Special Issue Polymer Hybrid Materials)
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Abstract

Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated. View Full-Text
Keywords: sortase-mediated ligation; enzymes; block copolymers; nanoparticles sortase-mediated ligation; enzymes; block copolymers; nanoparticles
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Dai, X.; Mate, D.M.; Glebe, U.; Mirzaei Garakani, T.; Körner, A.; Schwaneberg, U.; Böker, A. Sortase-Mediated Ligation of Purely Artificial Building Blocks. Polymers 2018, 10, 151.

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