Sortase-Mediated Ligation of Purely Artificial Building Blocks
1
Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam-Golm, Germany
2
Lehrstuhl für Polymermaterialien und Polymertechnologie, University of Potsdam, 14476 Potsdam-Golm, Germany
3
DWI—Leibniz Institute for Interactive Materials e.V., Forckenbeckstr. 50, 52056 Aachen, Germany
4
Institute of Biotechnology, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany
*
Authors to whom correspondence should be addressed.
†
Current address: Center of Molecular Biology “Severo Ochoa”, Universidad Autónoma de Madrid, Nicolás Cabrera 1, 28049 Madrid, Spain.
‡
These authors contributed equally to this work.
Polymers 2018, 10(2), 151; https://doi.org/10.3390/polym10020151
Received: 9 December 2017
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Revised: 19 January 2018
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Accepted: 2 February 2018
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Published: 6 February 2018
(This article belongs to the Special Issue Polymer Hybrid Materials)
Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.
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Keywords:
sortase-mediated ligation; enzymes; block copolymers; nanoparticles
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MDPI and ACS Style
Dai, X.; Mate, D.M.; Glebe, U.; Mirzaei Garakani, T.; Körner, A.; Schwaneberg, U.; Böker, A. Sortase-Mediated Ligation of Purely Artificial Building Blocks. Polymers 2018, 10, 151. https://doi.org/10.3390/polym10020151
AMA Style
Dai X, Mate DM, Glebe U, Mirzaei Garakani T, Körner A, Schwaneberg U, Böker A. Sortase-Mediated Ligation of Purely Artificial Building Blocks. Polymers. 2018; 10(2):151. https://doi.org/10.3390/polym10020151
Chicago/Turabian StyleDai, Xiaolin, Diana M. Mate, Ulrich Glebe, Tayebeh Mirzaei Garakani, Andrea Körner, Ulrich Schwaneberg, and Alexander Böker. 2018. "Sortase-Mediated Ligation of Purely Artificial Building Blocks" Polymers 10, no. 2: 151. https://doi.org/10.3390/polym10020151
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