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Polymers 2018, 10(10), 1073; https://doi.org/10.3390/polym10101073

Fabrication, Characterization, and Cytotoxicity of Thermoplastic Polyurethane/Poly(lactic acid) Material Using Human Adipose Derived Mesenchymal Stromal Stem Cells (hASCs)

1
AGH University of Science and Technology, Department of Biomaterials and Composites, Faculty of Materials Science and Science and Ceramics, Krakow 30-059, Poland
2
Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland
3
AGH University of Science and Technology, Faculty of Electrical Engineering, Automatics, Computer Science and Biomedical Engineering, Krakow 30-059, Poland
4
Faculty of Veterinary Medicine, Equine Clinic-Equine Surgery, Justus-Liebig-University, Gießen 35392, Germany
*
Author to whom correspondence should be addressed.
Received: 25 July 2018 / Revised: 24 September 2018 / Accepted: 25 September 2018 / Published: 28 September 2018
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Abstract

Thermoplastic polyurethane (TPU) and poly(lactic acid) are types of biocompatible and degradable synthetic polymers required for biomedical applications. Physically blended (TPU+PLA) tissue engineering matrices were produced via solvent casting technique. The following types of polymer blend were prepared: (TPU+PLA) 7:3, (TPU+PLA) 6:4, (TPU+PLA) 4:6, and (TPU+PLA) 3:7. Various methods were employed to characterize the properties of these polymers: surface properties such as morphology (scanning electron microscopy), wettability (goniometry), and roughness (profilometric analysis). Analyses of hydrophilic and hydrophobic properties, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) of the obtained polymer blends were conducted. Tensile tests demonstrated that the blends exhibited a wide range of mechanical properties. Cytotoxicity of polymers was tested using human multipotent stromal cells derived from adipose tissue (hASC). In vitro assays revealed that (TPU+PLA) 3:7 matrices were the most cytocompatible biomaterials. Cells cultured on (TPU+PLA) 3:7 had proper morphology, growth pattern, and were distinguished by increased proliferative and metabolic activity. Additionally, it appeared that (TPU+PLA) 3:7 biomaterials showed antiapoptotic properties. hASC cultured on these matrices had reduced expression of Bax-α and increased expression of Bcl-2. This study demonstrated the feasibility of producing a biocompatible scaffold form based on (TPU+PLA) blends that have potential to be applied in tissue engineering. View Full-Text
Keywords: thermoplastic polyurethane; poly(lactic acid), polymer blends; stem cells; osteochondral tissue engineering thermoplastic polyurethane; poly(lactic acid), polymer blends; stem cells; osteochondral tissue engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lis-Bartos, A.; Smieszek, A.; Frańczyk, K.; Marycz, K. Fabrication, Characterization, and Cytotoxicity of Thermoplastic Polyurethane/Poly(lactic acid) Material Using Human Adipose Derived Mesenchymal Stromal Stem Cells (hASCs). Polymers 2018, 10, 1073.

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