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Article

A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process

1
Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, 18071 Granada, Granada, Spain
2
Laboratory of Crystallographic Studies, Andalusian Institute of Earth Sciences, C.S.I.C. University of Granada, Avenida de las Palmeras No. 4, 18100 Armilla, Granada, Spain
3
Department of Organic Chemistry, University of Granada, 18071 Granada, Granada, Spain
*
Authors to whom correspondence should be addressed.
Academic Editors: Kyeong Kyu Kim and Dinadayalane Tandabany
Crystals 2022, 12(1), 18; https://doi.org/10.3390/cryst12010018
Received: 25 November 2021 / Revised: 8 December 2021 / Accepted: 21 December 2021 / Published: 23 December 2021
(This article belongs to the Special Issue State-of-the-Art Research in Biomolecular Crystals)
L-proline amide hydrolase (PAH, EC 3.5.1.101) is a barely described enzyme belonging to the peptidase S33 family, and is highly similar to prolyl aminopeptidases (PAP, EC. 3.4.11.5). Besides being an S-stereoselective character towards piperidine-based carboxamides, this enzyme also hydrolyses different L-amino acid amides, turning it into a potential biocatalyst within the Amidase Process. In this work, we report the characterization of L-proline amide hydrolase from Pseudomonas syringae (PsyPAH) together with the first X-ray structure for this class of L-amino acid amidases. Recombinant PsyPAH showed optimal conditions at pH 7.0 and 35 °C, with an apparent thermal melting temperature of 46 °C. The enzyme behaved as a monomer at the optimal pH. The L-enantioselective hydrolytic activity towards different canonical and non-canonical amino-acid amides was confirmed. Structural analysis suggests key residues in the enzymatic activity. View Full-Text
Keywords: amidase; amino acid; amidase process; proline; aminopeptidase; S33 family amidase; amino acid; amidase process; proline; aminopeptidase; S33 family
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MDPI and ACS Style

Martinez-Rodríguez, S.; Contreras-Montoya, R.; Torres, J.M.; de Cienfuegos, L.Á.; Gavira, J.A. A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process. Crystals 2022, 12, 18. https://doi.org/10.3390/cryst12010018

AMA Style

Martinez-Rodríguez S, Contreras-Montoya R, Torres JM, de Cienfuegos LÁ, Gavira JA. A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process. Crystals. 2022; 12(1):18. https://doi.org/10.3390/cryst12010018

Chicago/Turabian Style

Martinez-Rodríguez, Sergio, Rafael Contreras-Montoya, Jesús M. Torres, Luis Á. de Cienfuegos, and Jose A. Gavira. 2022. "A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process" Crystals 12, no. 1: 18. https://doi.org/10.3390/cryst12010018

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