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Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

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Instituto Politécnico Nacional-UPIIG, Av. Mineral de Valenciana #200, Fracc. Industrial Puerto Interior, Silao de la Victoria, Guanajuato 36275, Mexico
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Skolkovo Institute of Science and Technology, 3 Nobel Street, Moscow 121205, Russia
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NABICRON-Innovation Park AGROBIOTEG, Av. Camino Real de Guanajuato S/N, Ejido de Juarez, Irapuato, Guanajuato 36630, Mexico
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NANOBIOTECH A.C.-Innovation Park AGROBIOTEG, Av. Camino Real de Guanajuato S/N, Ejido de Juarez, Irapuato, Guanajuato 36630, Mexico
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Hospital Psiquiatico Fray Bernardino Alvarez, Av. San Buenaventura 2, Niño Jesús, Tlalpan, 14000 Ciudad de México, CDMX, Mexico
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Author to whom correspondence should be addressed.
Crystals 2020, 10(5), 395; https://doi.org/10.3390/cryst10050395
Received: 29 February 2020 / Revised: 1 April 2020 / Accepted: 3 April 2020 / Published: 15 May 2020
Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches. View Full-Text
Keywords: silver nanoparticles; green synthesis; antibacterial activity; Pseudomonas aeruginosa silver nanoparticles; green synthesis; antibacterial activity; Pseudomonas aeruginosa
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MDPI and ACS Style

Martínez Espinosa, J.C.; Carrera Cerritos, R.; Ramírez Morales, M.A.; Sánchez Guerrero, K.P.; Silva Contreras, R.A.; Macías, J.H. Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa. Crystals 2020, 10, 395.

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