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Catalysts 2018, 8(3), 96; https://doi.org/10.3390/catal8030096

X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production

1,2
,
1
,
1
and
1,*
1
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
2
Ministry of Science and Technology, Baghdad 10001, Iraq
*
Author to whom correspondence should be addressed.
Received: 25 January 2018 / Revised: 21 February 2018 / Accepted: 26 February 2018 / Published: 28 February 2018
(This article belongs to the Special Issue Immobilized Biocatalysts)
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Abstract

This study presents a one-step encapsulation method for synthesizing X-shaped zeolitic imidazolate frameworks (ZIF-8) and immobilizing Rhizomucor miehei lipase (RML). We proved that the morphological structure of ZIF-8 had changed after immobilization with enhanced characterization using a field-emission scanning electron microscope, an energy-dispersive spectrometer, a transmission electron microscope, a Fourier transform infrared spectrometer, and powder X-ray diffraction. The surface area and pore size of the carrier were investigated before and after immobilization using Brunauer–Emmett–Teller and Barrett–Joyner–Halenda methods, respectively. [email protected] exhibited high recovery activity of up to 2632%, representing a 26-fold increase in its free lipase. Encapsulated RML was used for biodiesel production from soybean oil in an isooctane system with a conversion yield of 95.6% under optimum conditions. The resulting reusability of the immobilized enzyme indicated no substantial decline in the conversion yield, which remained at 84.7% of the initial activity after 10 cycles. The stability and high performance of the immobilized enzyme are attributed to the harmony between RML and ZIF-8 based on the easy synthesis of ZIF-8 and the short time required to immobilize RML. View Full-Text
Keywords: X-shape ZIF-8; Rhizomucor miehei lipase (RML); encapsulation technique; biodiesel yield; soybean oil X-shape ZIF-8; Rhizomucor miehei lipase (RML); encapsulation technique; biodiesel yield; soybean oil
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Adnan, M.; Li, K.; Xu, L.; Yan, Y. X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production. Catalysts 2018, 8, 96.

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