PGC-1α Regulates Cell Proliferation, Migration, and Invasion by Modulating Leucyl-tRNA Synthetase 1 Expression in Human Colorectal Cancer Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Materials
2.3. RNA Isolation and First-Strand cDNA Synthesis
2.4. Annealing Control Primer (ACP)-Based GeneFishing PCR
2.5. Generation of Stable PGC-1α-Overexpressing SW480 and PGC-1α shRNA-Knocked Down SW620 Cell Line
2.6. Generation of Stable LARS1-Overexpressing SW480 and LARS1 shRNA-Knocked Down SW620 Cell Line
2.7. RNA Extraction and Real-Time Quantitative Reverse Transcriptase–Polymerase Chain Reaction (qRT-PCR)
2.8. Cell Counting
2.9. MTT Assay
2.10. Transwell Migration and Invasion Assays
2.11. Western Blot Analysis
2.12. Immunofluorescence Staining and Fluorescence Quantification
2.13. LARS1 Expression and LARS1 shRNA Expression Vector Transfection
2.14. PGC-1α Expression and PGC-1α shRNA Expression Vector Transfection
2.15. Statistical Analysis
3. Results
3.1. Overexpression of PGC-1α Leads to Upregulation of LARS1 in Human Embryonic Kidney 293 (HEK293) Cells
3.2. LARS1 Overexpression Enhances Cell Proliferation, Migration, and Invasion of SW480 Cells
3.3. LARS1 Knockdown Reduces Cell Proliferation, Migration, and Invasion of SW620 Cells
3.4. PGC-1α Regulates Cell Proliferation, Migration, and Invasion of PGC-1α-HEK293, SW480, and SW620 Cells by Regulating LARS1 Expression
3.5. PGC-1α Regulates Cell Proliferation, Migration, and Invasion through LARS1/AKT/GSK-3β/β-Catenin Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cho, J.G.; Park, S.-J.; Han, S.-H.; Park, J.-I. PGC-1α Regulates Cell Proliferation, Migration, and Invasion by Modulating Leucyl-tRNA Synthetase 1 Expression in Human Colorectal Cancer Cells. Cancers 2023, 15, 159. https://doi.org/10.3390/cancers15010159
Cho JG, Park S-J, Han S-H, Park J-I. PGC-1α Regulates Cell Proliferation, Migration, and Invasion by Modulating Leucyl-tRNA Synthetase 1 Expression in Human Colorectal Cancer Cells. Cancers. 2023; 15(1):159. https://doi.org/10.3390/cancers15010159
Chicago/Turabian StyleCho, Jun Gi, Su-Jeong Park, Sang-Heum Han, and Joo-In Park. 2023. "PGC-1α Regulates Cell Proliferation, Migration, and Invasion by Modulating Leucyl-tRNA Synthetase 1 Expression in Human Colorectal Cancer Cells" Cancers 15, no. 1: 159. https://doi.org/10.3390/cancers15010159