Evaluation of Comprehensive Gene Expression and NK Cell-Mediated Killing in Glioblastoma Cell Line-Derived Spheroids
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Method
2.1. Gene Expression Based on The Cancer Genome Atlas (TCGA) Data Set
2.2. Cell Lines
2.3. Spheroid Culture
2.4. Expansion of Primary Human NK Cells and Lymphocyte-Activated Killer Cells
2.5. CFSE-Based Cytotoxic Assay
2.6. Microarray Gene Expression Assay
2.7. Statistical Analysis
3. Results
3.1. Cancer Stem Cell Marker, ECM Marker, and Chemokine/Chemokine Receptor Expression in Human Glioma
3.2. Spheroid Formation and Comprehensive Gene Expression Analysis of 2D Adherent Cells vs. 3D Spheroids Derived from GBM Cell Lines
3.3. Comprehensive Gene Expression Analysis of the Expanded NK Cells as Compared to Conventional LAK Cells
3.4. Cytotoxicity Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Morimoto, T.; Nakazawa, T.; Matsuda, R.; Nishimura, F.; Nakamura, M.; Yamada, S.; Nakagawa, I.; Park, Y.-S.; Tsujimura, T.; Nakase, H. Evaluation of Comprehensive Gene Expression and NK Cell-Mediated Killing in Glioblastoma Cell Line-Derived Spheroids. Cancers 2021, 13, 4896. https://doi.org/10.3390/cancers13194896
Morimoto T, Nakazawa T, Matsuda R, Nishimura F, Nakamura M, Yamada S, Nakagawa I, Park Y-S, Tsujimura T, Nakase H. Evaluation of Comprehensive Gene Expression and NK Cell-Mediated Killing in Glioblastoma Cell Line-Derived Spheroids. Cancers. 2021; 13(19):4896. https://doi.org/10.3390/cancers13194896
Chicago/Turabian StyleMorimoto, Takayuki, Tsutomu Nakazawa, Ryosuke Matsuda, Fumihiko Nishimura, Mitsutoshi Nakamura, Shuichi Yamada, Ichiro Nakagawa, Young-Soo Park, Takahiro Tsujimura, and Hiroyuki Nakase. 2021. "Evaluation of Comprehensive Gene Expression and NK Cell-Mediated Killing in Glioblastoma Cell Line-Derived Spheroids" Cancers 13, no. 19: 4896. https://doi.org/10.3390/cancers13194896