O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Differential Glycomic Profiling of Specific EVs in PC Sera Using Lectin Microarray
2.2. The ABA- and ACA-Positive Glycans are Strongly Localized in the Pathological Lesion of PC
2.3. Quantification of the ABA- and ACA-Positive EVs Derived from Cancer Cell Lines with ExoCounter
2.4. The Numbers of ABA- and ACA-Positive EVs were Elevated in Sera from PC Patients
2.5. Evaluation of the Diagnostic Utility of ABA- and ACA-Positive EV Counts Elevated in PC Sera
3. Discussion
4. Materials and Methods
4.1. Antibody and Lectin
4.2. Cell Culture
4.3. Clinical Samples
4.4. Analysis with Lectin Microarray
4.5. Lectin-Staining of PC Tissue Sections
4.6. Preparation of Antibody-Conjugated Nanobeads
4.7. EV Quantification with ExoCounter
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lectins 1 | Study 1 | Study 2 | ||||
---|---|---|---|---|---|---|
Tumor/Normal Ratio | p Value 2 | Pre-/Post-Operative Ratio | ||||
DSA | 1.375 | <0.001 | 3 | 4 | 0.953 | |
STL | 1.440 | <0.001 | 3 | 4 | 0.822 | |
LEL | 1.276 | 0.001 | 3 | 4 | 0.866 | |
UDA | 1.170 | 0.011 | 3 | 4 | 0.780 | |
ACA | 1.199 | 0.058 | 3 | 4 | 1.175 | 6 |
ABA | 1.239 | 0.063 | 3 | 4 | 1.387 | 6 |
SSA | 0.820 | <0.001 | 3 | 5 | 1.097 | |
TxLC I | 0.764 | 0.009 | 3 | 5 | 1.019 | |
Calsepa | 0.800 | 0.012 | 3 | 5 | 1.054 | |
PHA(E) | 0.814 | 0.085 | 5 | 0.959 | ||
ACG | 0.899 | 0.157 | ||||
LCA | 0.898 | 0.186 | ||||
WGA | 1.131 | 0.322 | ||||
AAL | 1.119 | 0.443 |
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Yokose, T.; Kabe, Y.; Matsuda, A.; Kitago, M.; Matsuda, S.; Hirai, M.; Nakagawa, T.; Masugi, Y.; Hishiki, T.; Nakamura, Y.; et al. O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer. Cancers 2020, 12, 2469. https://doi.org/10.3390/cancers12092469
Yokose T, Kabe Y, Matsuda A, Kitago M, Matsuda S, Hirai M, Nakagawa T, Masugi Y, Hishiki T, Nakamura Y, et al. O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer. Cancers. 2020; 12(9):2469. https://doi.org/10.3390/cancers12092469
Chicago/Turabian StyleYokose, Takahiro, Yasuaki Kabe, Atsushi Matsuda, Minoru Kitago, Sachiko Matsuda, Miwa Hirai, Tomomi Nakagawa, Yohei Masugi, Takako Hishiki, Yuki Nakamura, and et al. 2020. "O-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer" Cancers 12, no. 9: 2469. https://doi.org/10.3390/cancers12092469