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Rescue of Non-Informative Circulating Tumor DNA to Monitor the Mutational Landscape in NSCLC

1
Institute of Pathology, University Medical Center Ulm, 89070 Ulm, Germany
2
Department of Internal Medicine II, University Medical Center Ulm, 89070 Ulm, Germany
3
Institute of Experimental Tumor Research, University Medical Center Ulm, 89070 Ulm, Germany
4
Department of Internal Medicine III, University Medical Center Ulm, 89070 Ulm, Germany
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(7), 1917; https://doi.org/10.3390/cancers12071917
Received: 11 June 2020 / Revised: 10 July 2020 / Accepted: 14 July 2020 / Published: 16 July 2020
(This article belongs to the Section Methods and Technologies Development)
In non-small cell lung cancer (NSCLC) the usage of plasma-derived circulating tumor DNA (ctDNA) have come into focus to obtain a comprehensive genetic profile of a given lung cancer. Despite the usage of specific sampling tubes, archived plasma samples as well as inappropriately treated blood samples still cause a loss of information due to cell lysis and contamination with cellular DNA. Our aim was to establish a reliable protocol to rescue ctDNA from such non-informative samples to monitor the mutational landscape in NSCLC. As a proof-of-concept study we used archived plasma samples derived from whole blood EDTA samples of 51 patients suffering from NSCLC. Analysis of the isolated plasma DNA determined only a small fraction of ctDNA in a range of 90–250 bp. By applying a specific purification procedure, we were able to increase the informative ctDNA content and improve in a cohort of 42 patients the detection of driver mutations from 32% to 79% of the mutations found in tissue biopsies. Thus, we present here an easy to perform, time and cost effective procedure to rescue non-informative ctDNA samples, which is sufficient to detect oncogenic mutations in NGS approaches and is therefore a valuable technical improvement for laboratories handling liquid biopsy samples. View Full-Text
Keywords: liquid biopsy; cell-free DNA; NGS; precision medicine; targeted therapy liquid biopsy; cell-free DNA; NGS; precision medicine; targeted therapy
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MDPI and ACS Style

Mayer, S.; Schmidtke-Schrezenmeier, G.; Buske, C.; Rücker, F.G.; Barth, T.F.E.; Möller, P.; Marienfeld, R. Rescue of Non-Informative Circulating Tumor DNA to Monitor the Mutational Landscape in NSCLC. Cancers 2020, 12, 1917. https://doi.org/10.3390/cancers12071917

AMA Style

Mayer S, Schmidtke-Schrezenmeier G, Buske C, Rücker FG, Barth TFE, Möller P, Marienfeld R. Rescue of Non-Informative Circulating Tumor DNA to Monitor the Mutational Landscape in NSCLC. Cancers. 2020; 12(7):1917. https://doi.org/10.3390/cancers12071917

Chicago/Turabian Style

Mayer, Stefanie, Gerlinde Schmidtke-Schrezenmeier, Christian Buske, Frank G. Rücker, Thomas F.E. Barth, Peter Möller, and Ralf Marienfeld. 2020. "Rescue of Non-Informative Circulating Tumor DNA to Monitor the Mutational Landscape in NSCLC" Cancers 12, no. 7: 1917. https://doi.org/10.3390/cancers12071917

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