BAGE Hypomethylation Is an Early Event in Colon Transformation and Is Frequent in Histologically Advanced Adenomas
Abstract
:1. Introduction
2. Results and Discussion
2.1. BAGE loci Are Hypomethylated in Colorectal Adenomas
Samples | Sex | Age | Site | Histological type | Grade of dysplasia | % precancerous cells | % DNA methylation† |
---|---|---|---|---|---|---|---|
T1 | M | 62 | Right colon | T | LG | 60 | 62 |
T3 | M | 75 | Sigmoid | TV | LG | 100 | 52 |
T4* | F | 69 | Right colon | TV | LG | 100 | 0 |
T5* | F | 69 | Right colon | TV | LG | 100 | 24 |
T6‡ | M | 70 | Sigmoid | V | LG | 100 | 63 |
T7‡ | M | 70 | Sigmoid | TV | LG | 100 | 66 |
T10 | M | 56 | Right colon | TV | LG | 60 | 97 |
T11 | M | 63 | Left colon | TV | LG | 100 | 11 |
T12 | F | 51 | Rectum | V | LG | 80 | 0 |
T13 | F | 62 | Left colon | T | LG | 100 | 83 |
T14 | F | 19 | Coloproctectomy | T | LG | 100 | 54 |
T15 | F | 19 | Coloproctectomy | TV | LG | 100 | 2 |
T16 | M | 60 | Sigmoid | T | LG | 100 | 47 |
T17 | F | 56 | Coloproctectomy | T | LG | 100 | 98 |
T18 | M | 70 | Right colon | T | LG | 100 | 48 |
T19 | M | 67 | Coloproctectomy | V | LG and HG | 100 | 75 |
T20 | M | 59 | Coloproctectomy | T | LG | 100 | 15 |
M21 | F | 56 | Colon | V | LG | 90 | 57 |
M22 | F | 58 | Colon | TV | LG | 85 | 52 |
M24 | M | 60 | Colon | TV | HG | 90 | 31 |
M25 | M | 78 | Colon | T | LG | 85 | 48 |
M26 | M | 61 | Rectum | V | LG | 90 | 33 |
M27 | M | 70 | Colon | V | HG | 90 | 24 |
M40 | F | 21 | Colon | T | LG | 90 | 26 |
K3 | M | 80 | Rectum | – | LG | n.d. | 28 |
K4 | F | 56 | Rectum | V | LG | n.d. | 26 |
K8 | M | 63 | Rectum | – | LG | n.d. | 6 |
K9a# | F | 48 | Rectum | – | LG | n.d. | 50 |
K9b# | F | 48 | Rectum | – | LG | n.d. | 16 |
K10 | M | 61 | Rectum | – | HG | n.d. | 49 |
K11 | M | 63 | n.d. | – | LG | n.d. | 102 |
K13 | M | 39 | Rectum | – | HG | n.d. | 59 |
K14 | M | 84 | Rectum | V | HG | n.d. | 86 |
K15 | M | 53 | Rectum | – | LG | n.d. | 40 |
K16 | M | 46 | Rectum | – | LG | n.d. | 93 |
K17 | M | 67 | Rectum | – | LG | n.d. | 30 |
K18 | M | 72 | Rectum | – | LG | n.d. | 37 |
K19 | M | 58 | Rectum | – | LG | n.d. | 43 |
K20 | M | 28 | Rectum | – | HG | n.d. | 82 |
K21 | F | 56 | Sigmoid | V | LG | n.d. | 64 |
K22 | F | 37 | Rectum | V | LG | n.d. | 100 |
K23 | M | 59 | Sigmoid | V | LG | n.d. | 21 |
K24 | F | 73 | Sigmoid | V | LG | n.d. | 72 |
K25 | F | 77 | Rectum | V | LG | n.d. | 35 |
2.2. Hypomethylation of BAGE Loci and Histological Features
Tissues | Frequencies | Min% (2) | Max% (2) |
---|---|---|---|
mucosas (1) | (3/54) 5.6% | 1.2 | 15.4 |
cancers (1) | (44/53) 83.0% | 70.2 | 91.9 |
adenomas | (19/44) 43.2% | 28.3 | 59.0 |
T adenomas | (2/9) 22.2% | 2.8 | 60.0 |
TV + V adenomas | (11/22) 50.0% | 28.2 | 71.8 |
Tissues | p-Values |
---|---|
adenomas vs mucosas | 7 × 10−5 |
cancers vs adenomas | 1.4 × 10−6 |
TV+V adenomas vs mucosas | 2.7 × 10−5 |
TV+V adenomas vs cancers | 4.7 × 10−3 |
T adenomas vs mucosas | 0.15 |
T adenomas vs cancers | 6.1 × 10−4 |
TV+V adenomas vs T adenomas | 0.15 |
3. Experimental Section
3.1.DNA Samples
3.2.DNA Methylation Analysis
3.3. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Lana, E.; Brun, M.-E.; Rivals, I.; Selves, J.; Kirzin, S.; Lutsyk, A.P.; Gordiyuk, V.V.; Bibeau, F.; Rynditch, A.; De Sario, A. BAGE Hypomethylation Is an Early Event in Colon Transformation and Is Frequent in Histologically Advanced Adenomas. Cancers 2009, 1, 3-11. https://doi.org/10.3390/cancers1010003
Lana E, Brun M-E, Rivals I, Selves J, Kirzin S, Lutsyk AP, Gordiyuk VV, Bibeau F, Rynditch A, De Sario A. BAGE Hypomethylation Is an Early Event in Colon Transformation and Is Frequent in Histologically Advanced Adenomas. Cancers. 2009; 1(1):3-11. https://doi.org/10.3390/cancers1010003
Chicago/Turabian StyleLana, Erica, Marie-Elisabeth Brun, Isabelle Rivals, Janick Selves, Sylvain Kirzin, Andriy P. Lutsyk, Vasily V. Gordiyuk, Frédéric Bibeau, Alla Rynditch, and Albertina De Sario. 2009. "BAGE Hypomethylation Is an Early Event in Colon Transformation and Is Frequent in Histologically Advanced Adenomas" Cancers 1, no. 1: 3-11. https://doi.org/10.3390/cancers1010003
APA StyleLana, E., Brun, M.-E., Rivals, I., Selves, J., Kirzin, S., Lutsyk, A. P., Gordiyuk, V. V., Bibeau, F., Rynditch, A., & De Sario, A. (2009). BAGE Hypomethylation Is an Early Event in Colon Transformation and Is Frequent in Histologically Advanced Adenomas. Cancers, 1(1), 3-11. https://doi.org/10.3390/cancers1010003