Micromachines 2013, 4(3), 333-356; doi:10.3390/mi4030333
Review

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

1 Institute of Nano Engineering and Microsystems, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan 2 Department of Engineering and System Science, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan 3 Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Nankang, 115 Taipei, Taiwan
* Author to whom correspondence should be addressed.
Received: 24 June 2013; in revised form: 1 August 2013 / Accepted: 20 August 2013 / Published: 6 September 2013
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
PDF Full-text Download PDF Full-Text [4447 KB, Updated Version, uploaded 9 September 2013 16:17 CEST]
The original version is still available [4980 KB, uploaded 6 September 2013 14:15 CEST]
Abstract: The behaviors of cell to cell or cell to environment with their organelles and their intracellular physical or biochemical effects are still not fully understood. Analyzing millions of cells together cannot provide detailed information, such as cell proliferation, differentiation or different responses to external stimuli and intracellular reaction. Thus, single cell level research is becoming a pioneering research area that unveils the interaction details in high temporal and spatial resolution among cells. To analyze the cellular function, single cell electroporation can be conducted by employing a miniaturized device, whose dimension should be similar to that of a single cell. Micro/nanofluidic devices can fulfill this requirement for single cell electroporation. This device is not only useful for cell lysis, cell to cell fusion or separation, insertion of drug, DNA and antibodies inside single cell, but also it can control biochemical, electrical and mechanical parameters using electroporation technique. This device provides better performance such as high transfection efficiency, high cell viability, lower Joule heating effect, less sample contamination, lower toxicity during electroporation experiment when compared to bulk electroporation process. In addition, single organelles within a cell can be analyzed selectively by reducing the electrode size and gap at nanoscale level. This advanced technique can deliver (in/out) biomolecules precisely through a small membrane area (micro to nanoscale area) of the single cell, known as localized single cell membrane electroporation (LSCMEP). These articles emphasize the recent progress in micro/nanofluidic single cell electroporation, which is potentially beneficial for high-efficient therapeutic and delivery applications or understanding cell to cell interaction.
Keywords: bulk electroporation (BEP); single cell electroporation (SCEP); localized single cell membrane electroporation (LSCMEP); cell transfection; cell lysis

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Santra, T.S.; Tseng, F.G. Recent Trends on Micro/Nanofluidic Single Cell Electroporation. Micromachines 2013, 4, 333-356.

AMA Style

Santra TS, Tseng FG. Recent Trends on Micro/Nanofluidic Single Cell Electroporation. Micromachines. 2013; 4(3):333-356.

Chicago/Turabian Style

Santra, Tuhin S.; Tseng, Fang G. 2013. "Recent Trends on Micro/Nanofluidic Single Cell Electroporation." Micromachines 4, no. 3: 333-356.

Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert