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A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange

Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan
School of Integrated Design Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Present address: Division of Molecular Science, Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
Present address: School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Micromachines 2020, 11(12), 1123;
Received: 30 November 2020 / Revised: 16 December 2020 / Accepted: 17 December 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Lipid Bilayers on Chip)
Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurements using a pumpless solution exchange procedure. The device consists of a millimeter-scale cylindrical cup with vertical slits which is designed to steadily hold an aqueous solution and exchange the sample by simply fusing and splitting the solution with an external solution. The slit design was experimentally determined by the capabilities of both the retention and exchange of the solution. Using the optimized slit, a planar lipid bilayer was reconstituted with a nanopore protein at a microaperture allocated to the bottom of the cup, and the device was connected to a portable amplifier. The solution exchangeability was demonstrated by observing the dilution process of a blocker molecule of the nanopore dissolved in the cup. The pumpless solution exchange by the proposed cup-like device presents potential as a lipid-bilayer system for portable sensing applications. View Full-Text
Keywords: lipid-bilayer membrane; sample exchange; nanopore sensor; portable sensor lipid-bilayer membrane; sample exchange; nanopore sensor; portable sensor
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MDPI and ACS Style

Ito, Y.; Izawa, Y.; Osaki, T.; Kamiya, K.; Misawa, N.; Fujii, S.; Mimura, H.; Miki, N.; Takeuchi, S. A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange. Micromachines 2020, 11, 1123.

AMA Style

Ito Y, Izawa Y, Osaki T, Kamiya K, Misawa N, Fujii S, Mimura H, Miki N, Takeuchi S. A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange. Micromachines. 2020; 11(12):1123.

Chicago/Turabian Style

Ito, Yoshihisa, Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Nobuo Misawa, Satoshi Fujii, Hisatoshi Mimura, Norihisa Miki, and Shoji Takeuchi. 2020. "A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange" Micromachines 11, no. 12: 1123.

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