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Open AccessArticle

Humoral Response of Buffaloes to a Recombinant Vaccine against Botulism Serotypes C and D

Instituto de Medicina Veterinária, Universidade Federal do Pará, BR 316 Km 61, Saudade II, Cristo Redentor, Castanhal 68740-910, PA, Brazil
Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil
Author to whom correspondence should be addressed.
Academic Editors: Jianlong Lou and James D. Marks
Toxins 2017, 9(10), 297;
Received: 31 August 2017 / Revised: 15 September 2017 / Accepted: 16 September 2017 / Published: 22 September 2017
(This article belongs to the Special Issue Botulinum Neurotoxins Antibody and Vaccine)
Botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs), which are mainly produced by Clostridium botulinum and characterized by flaccid paralysis. The BoNTs C and D are the main serotypes responsible for botulism in animals, including buffaloes. Botulism is one of the leading causes of death in adult ruminants in Brazil due to the high mortality rates, even though botulism in buffaloes is poorly reported and does not reflect the real economic impact of this disease in Brazilian herds. Vaccination is reported as the most important prophylactic measure for botulism control, although there are no specific vaccines commercially available for buffaloes in Brazil. This study aimed to evaluate the humoral immune response of buffalo groups vaccinated with three different concentrations of recombinant proteins (100, 200, and 400 µg) against BoNTs serotypes C and D as well as to compare the groups to each other and with a group vaccinated with a bivalent commercial toxoid. The recombinant vaccine with a concentration of 400 μg of proteins induced the highest titers among the tested vaccines and was proven to be the best choice among the formulations evaluated and should be considered as a potential vaccine against botulism in buffalo. View Full-Text
Keywords: Clostridium botulinum; botulinum neurotoxins; BoNT; antitoxins Clostridium botulinum; botulinum neurotoxins; BoNT; antitoxins
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Otaka, D.Y.; Barbosa, J.D.; Moreira, C., Jr.; Ferreira, M.R.A.; Cunha, C.E.P.; Brito, A.R.S.; Donassolo, R.A.; Moreira, Â.N.; Conceição, F.R.; Salvarani, F.M. Humoral Response of Buffaloes to a Recombinant Vaccine against Botulism Serotypes C and D. Toxins 2017, 9, 297.

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