Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus
Abstract
:1. Introduction
2. Results
2.1. Effect of Zinc Chelators, TPEN and DMPS on Aflatoxin Accumulation and Growth of A. parasiticus
2.2. Effect of TPEN on Aflatoxin Transcript Accumulation in A. parasiticus SU-1
2.3. TPEN Increases Intracellular Zinc Levels
2.4. TPEN Affects Toxin Accumulation in A. parasiticus Grown on Aflatoxin-Inducing Peanut-Derived Medium but Not in Sunflower Seed-Derived Medium
3. Discussion
4. Conclusion and Future Perspectives
5. Material and Methods
5.1. Fungal Strains and Growth Conditions
5.2. Aflatoxins B1, B2, G1, and G2 and Norsolorinic Acid Extraction, Detection, and Analysis
5.3. Total RNA Extraction and Gene Expression Analysis
5.4. Zinpyr-1 and Confocal Scanning Laser Microscopy
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gene | Primer Sequence | Reference |
---|---|---|
aflR | F-5′ TGAGAACGATAAGGACGAC 3′ | [4] |
R-5′ CATCCTCAATCGAATCAAC 3′ | ||
ver-1 | F-5′ TTGTATCGTTCGGTCACC 3′ | [45] |
R-3′ GGTTCAAAGGAGAGAGCC 3′ | ||
citrate synthase | F-5′ TGCAGTCCGTTGCCTTCAATG 3′ | [5] |
R-5′ TAGCGTAGGCCTTGGCGAAAG 3′ | ||
pksA | F-5′ GTTCCTTGGCCGCTGTG 3′ | [45] |
R-5′ AAAGGCGTGGCAGTCC 3′ |
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Wee, J.; Day, D.M.; Linz, J.E. Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus. Toxins 2016, 8, 171. https://doi.org/10.3390/toxins8060171
Wee J, Day DM, Linz JE. Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus. Toxins. 2016; 8(6):171. https://doi.org/10.3390/toxins8060171
Chicago/Turabian StyleWee, Josephine, Devin M. Day, and John E. Linz. 2016. "Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus" Toxins 8, no. 6: 171. https://doi.org/10.3390/toxins8060171