Next Article in Journal
Comment on Detection of Mycotoxins in Patients with Chronic Fatigue Syndrome. Toxins 2013, 5, 605–617
Previous Article in Journal
Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Toxins 2016, 8(11), 320;

Screening of Cytotoxic B. cereus on Differentiated Caco-2 Cells and in Co-Culture with Mucus-Secreting (HT29-MTX) Cells

Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, Croix du Sud 2, L7.05.12, Louvain-la-Neuve B-1348, Belgium
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université catholique de Louvain, Croix du Sud 4-5, L7.07.03, Louvain-la-Neuve B-1348 , Belgium
Author to whom correspondence should be addressed.
Academic Editor: Yukako Fujinaga
Received: 1 August 2016 / Revised: 9 October 2016 / Accepted: 31 October 2016 / Published: 5 November 2016
(This article belongs to the Section Bacterial Toxins)
Full-Text   |   PDF [1351 KB, uploaded 5 November 2016]   |  


B. cereus is an opportunistic foodborne pathogen able to cause diarrhoea. However, the diarrhoeal potential of a B. cereus strain remains difficult to predict, because no simple correlation has yet been identified between the symptoms and a unique or a specific combination of virulence factors. In this study, 70 B. cereus strains with different origins (food poisonings, foods and environment) have been selected to assess their enterotoxicity. The B. cereus cell-free supernatants have been tested for their toxicity in vitro, on differentiated (21 day-old) Caco-2 cells, using their ATP content, LDH release and NR accumulation. The genetic determinants of the main potential enterotoxins and virulence factors (ces, cytK, entFM, entS, hbl, nhe, nprA, piplC and sph) have also been screened by PCR. This analysis showed that none of these genes was able to fully explain the enterotoxicity of B. cereus strains. Additionally, in order to assess a possible effect of the mucus layer in vitro, a cytotoxicity comparison between a monoculture (Caco-2 cells) and a co-culture (Caco-2 and HT29-MTX mucus-secreting cells) model has been performed with selected B. cereus supernatants. It appeared that, in these conditions, the mucus layer had no notable influence on the cytotoxicity of B. cereus supernatants. View Full-Text
Keywords: B. cereus; enterotoxin; cytotoxicity; Caco-2; HT29-MTX B. cereus; enterotoxin; cytotoxicity; Caco-2; HT29-MTX

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Castiaux, V.; Laloux, L.; Schneider, Y.-J.; Mahillon, J. Screening of Cytotoxic B. cereus on Differentiated Caco-2 Cells and in Co-Culture with Mucus-Secreting (HT29-MTX) Cells. Toxins 2016, 8, 320.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top