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Open AccessArticle

Development and Evaluation of Monoclonal Antibodies for Paxilline

Bacterial Foodborne Pathogens and Mycology Research Unit, USDA-ARS-NCAUR, 1815 North University Street, Peoria, IL 61604, USA
Academic Editor: Laura Anfossi
Toxins 2015, 7(10), 3903-3915; https://doi.org/10.3390/toxins7103903
Received: 14 August 2015 / Revised: 8 September 2015 / Accepted: 22 September 2015 / Published: 25 September 2015
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
Paxilline (PAX) is a tremorgenic mycotoxin that has been found in perennial ryegrass infected with Acremonium lolii. To facilitate screening for this toxin, four murine monoclonal antibodies (mAbs) were developed. In competitive indirect enzyme-linked immunosorbent assays (CI-ELISAs) the concentrations of PAX required to inhibit signal development by 50% (IC50s) ranged from 1.2 to 2.5 ng/mL. One mAb (2-9) was applied to the detection of PAX in maize silage. The assay was sensitive to the effects of solvents, with 5% acetonitrile or 20% methanol causing a two-fold or greater increase in IC50. For analysis of silage samples, extracts were cleaned up by adsorbing potential matrix interferences onto a solid phase extraction column. The non-retained extract was then diluted with buffer to reduce solvent content prior to assay. Using this method, the limit of detection for PAX in dried silage was 15 µg/kg and the limit of quantification was 90 µg/kg. Recovery from samples spiked over the range of 100 to 1000 µg/kg averaged 106% ± 18%. The assay was applied to 86 maize silage samples, with many having detectable, but none having quantifiable, levels of PAX. The results suggest the CI-ELISA can be applied as a sensitive technique for the screening of PAX in maize silage. View Full-Text
Keywords: paxilline; tremorgen; antibody; immunoassay; mycotoxin; silage paxilline; tremorgen; antibody; immunoassay; mycotoxin; silage
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MDPI and ACS Style

Maragos, C.M. Development and Evaluation of Monoclonal Antibodies for Paxilline. Toxins 2015, 7, 3903-3915.

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