Toxins 2014, 6(3), 1049-1061; https://doi.org/10.3390/toxins6031049
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB
1
College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
2
Department of Biological Sciences, School of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UK
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 20 December 2013 / Revised: 26 February 2014 / Accepted: 3 March 2014 / Published: 12 March 2014
(This article belongs to the Section Bacterial Toxins)
Abstract
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis. View Full-TextKeywords:
NetB; pore-forming toxin; Clostridium perfringens; necrotic enteritis
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Fernandes da Costa, S.P.; Savva, C.G.; Bokori-Brown, M.; Naylor, C.E.; Moss, D.S.; Basak, A.K.; Titball, R.W. Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB. Toxins 2014, 6, 1049-1061.