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Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

1
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China
2
College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
3
Institute of Quality Standards and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
4
Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 214122, China
*
Author to whom correspondence should be addressed.
Toxins 2022, 14(5), 343; https://doi.org/10.3390/toxins14050343
Received: 12 April 2022 / Revised: 3 May 2022 / Accepted: 12 May 2022 / Published: 16 May 2022
Ochratoxin A (OTA) is a mycotoxin that can contaminate a wide range of crops such as grains and grapes. In this study, a novel fungal mutant strain (FS-UV-21) with a high OTA degradation rate (74.5%) was obtained from Aspergillus niger irradiated with ultraviolet light (15 W for 20 min). The effect of pH, temperature, and inoculation concentration on the degradation of OTA by FS-UV-21 was investigated, and the results revealed that the detoxification effect was optimal (89.4%) at a pH of 8 and a temperature of 30 °C. Ultra-performance liquid chromatography-tandem mass spectrometry was used to characterize the degraded products of OTA, and the main degraded product was ochratoxin α. Triple quadrupole-linear ion trap-mass spectrometry combined with LightSight software was used to analyze the biotransformation pathway of OTA in FS-UV-21, to trace the degraded products, and to identify the main metabolite, P1 (C19H18ClNO6, m/z 404). After the FS-UV-21 strain was treated with OTA, the HepG2 cellular toxicity of the degradation products was significantly reduced. For the real sample, FS-UV-21 was used to remove OTA from wheat bran contaminated by mycotoxins through fermentation, resulting in the degradation of 59.8% of OTA in wheat bran. Therefore, FS-UV-21 can be applied to the degradation of OTA in agricultural products and food. View Full-Text
Keywords: Ochratoxin A; detoxification; Aspergillus niger; degradation products; cytotoxicity Ochratoxin A; detoxification; Aspergillus niger; degradation products; cytotoxicity
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MDPI and ACS Style

Zou, D.; Ji, J.; Ye, Y.; Yang, Y.; Yu, J.; Wang, M.; Zheng, Y.; Sun, X. Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain. Toxins 2022, 14, 343. https://doi.org/10.3390/toxins14050343

AMA Style

Zou D, Ji J, Ye Y, Yang Y, Yu J, Wang M, Zheng Y, Sun X. Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain. Toxins. 2022; 14(5):343. https://doi.org/10.3390/toxins14050343

Chicago/Turabian Style

Zou, Dong, Jian Ji, Yongli Ye, Yang Yang, Jian Yu, Meng Wang, Yi Zheng, and Xiulan Sun. 2022. "Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain" Toxins 14, no. 5: 343. https://doi.org/10.3390/toxins14050343

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