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Article

In Vitro Biological Control of Aspergillus flavus by Hanseniaspora opuntiae L479 and Hanseniaspora uvarum L793, Producers of Antifungal Volatile Organic Compounds

1
Food Quality and Microbiology, School of Agricultural Engineering, University of Extremadura, Avda. de Adolfo Suárez, s/n, 06007 Badajoz, Spain
2
University Institute for Research in Agri-Food Resources (INURA), University of Extremadura, Avda. de la Investigación, s/n, 06006 Badajoz, Spain
3
Finca La Orden-Valdesequera Research Centre (CICYTEX), Horticulture, Junta de Extremadura, 06187 Badajoz, Spain
*
Author to whom correspondence should be addressed.
Toxins 2021, 13(9), 663; https://doi.org/10.3390/toxins13090663
Received: 9 August 2021 / Revised: 28 August 2021 / Accepted: 8 September 2021 / Published: 17 September 2021
Aspergillus flavus is a toxigenic fungal colonizer of fruits and cereals and may produce one of the most important mycotoxins from a food safety perspective, aflatoxins. Therefore, its growth and mycotoxin production should be effectively avoided to protect consumers’ health. Among the safe and green antifungal strategies that can be applied in the field, biocontrol is a recent and emerging strategy that needs to be explored. Yeasts are normally good biocontrol candidates to minimize mold-related hazards and their modes of action are numerous, one of them being the production of volatile organic compounds (VOCs). To this end, the influence of VOCs produced by Hanseniaspora opuntiae L479 and Hanseniaspora uvarum L793 on growth, expression of the regulatory gene of the aflatoxin pathway (aflR) and mycotoxin production by A. flavus for 21 days was assessed. The results showed that both yeasts, despite producing different kinds of VOCs, had a similar effect on inhibiting growth, mycotoxin biosynthetic gene expression and phenotypic toxin production overall at the mid-incubation period when their synthesis was the greatest. Based on the results, both yeast strains, H. opuntiae L479 and H. uvarum L793, are potentially suitable as a biopreservative agents for inhibiting the growth of A. flavus and reducing aflatoxin accumulation. View Full-Text
Keywords: relative gene expression; aflatoxin production; antagonism; antifungal volatile esters relative gene expression; aflatoxin production; antagonism; antifungal volatile esters
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MDPI and ACS Style

Tejero, P.; Martín, A.; Rodríguez, A.; Galván, A.I.; Ruiz-Moyano, S.; Hernández, A. In Vitro Biological Control of Aspergillus flavus by Hanseniaspora opuntiae L479 and Hanseniaspora uvarum L793, Producers of Antifungal Volatile Organic Compounds. Toxins 2021, 13, 663. https://doi.org/10.3390/toxins13090663

AMA Style

Tejero P, Martín A, Rodríguez A, Galván AI, Ruiz-Moyano S, Hernández A. In Vitro Biological Control of Aspergillus flavus by Hanseniaspora opuntiae L479 and Hanseniaspora uvarum L793, Producers of Antifungal Volatile Organic Compounds. Toxins. 2021; 13(9):663. https://doi.org/10.3390/toxins13090663

Chicago/Turabian Style

Tejero, Paula, Alberto Martín, Alicia Rodríguez, Ana I. Galván, Santiago Ruiz-Moyano, and Alejandro Hernández. 2021. "In Vitro Biological Control of Aspergillus flavus by Hanseniaspora opuntiae L479 and Hanseniaspora uvarum L793, Producers of Antifungal Volatile Organic Compounds" Toxins 13, no. 9: 663. https://doi.org/10.3390/toxins13090663

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