Next Article in Journal
Cyanotoxins and the Nervous System
Previous Article in Journal
Cadherin Protein Is Involved in the Action of Bacillus thuringiensis Cry1Ac Toxin in Ostrinia furnacalis
Article

Validation of LC-MS/MS Coupled with a Chiral Column for the Determination of 3- or 15-Acetyl Deoxynivalenol Mycotoxins from Fusarium graminearum in Wheat

1
SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200030, China
2
Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affiairs, Urumqi 830091, China
3
Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
4
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Toxins 2021, 13(9), 659; https://doi.org/10.3390/toxins13090659
Received: 9 August 2021 / Revised: 3 September 2021 / Accepted: 10 September 2021 / Published: 16 September 2021
(This article belongs to the Special Issue Mycotoxins Study: Identification and Control)
The major causal agents Fusarium graminearum (F. graminearum) and Fusarium asiaticum could produce multiple mycotoxins in infected wheat, which threatens the health of humans and animals. Specifically, deoxynivalenol (DON) and its derivatives 3- and 15-acetyldeoxynivalenol (3-ADON and 15-ADON) are commonly detected mycotoxins in cereal grains. However, the good chromatographic separation of 3-ADON and 15-ADON remains challenging. Here, an LC-MS/MS method for the chemotype determination of Fusarium strains was developed and validated. 3- and 15-ADON could be separated chromatographically in this study with sufficiently low limits of detection (LODs; 4 μg/kg) and limits of quantification (LOQs; 8 μg/kg). The satisfying intraday and interday reproducibility (both %RSDr and %RSDR were <20%) of this method indicated good stability. The recoveries of all analytes were in the range of 80–120%. In addition, three F. graminearum complex (FGC) strains, i.e., PH-1 (chemotype 15-ADON), F-1 (chemotype 3-ADON) and 5035 (chemotype 15-ADON), were selected to verify the accuracy of the method in differentiating phenotypes. The validation results showed that this LC-MS/MS method based on sample pretreatment is effective and suitable for the chromatographic separation of 3-ADON and 15-ADON in wheat. View Full-Text
Keywords: chromatographic separation; chemotype determination; mycotoxin; wheat chromatographic separation; chemotype determination; mycotoxin; wheat
Show Figures

Figure 1

MDPI and ACS Style

Wang, L.; Yan, Z.; Zhou, H.; Fan, Y.; Wang, C.; Zhang, J.; Liao, Y.; Wu, A. Validation of LC-MS/MS Coupled with a Chiral Column for the Determination of 3- or 15-Acetyl Deoxynivalenol Mycotoxins from Fusarium graminearum in Wheat. Toxins 2021, 13, 659. https://doi.org/10.3390/toxins13090659

AMA Style

Wang L, Yan Z, Zhou H, Fan Y, Wang C, Zhang J, Liao Y, Wu A. Validation of LC-MS/MS Coupled with a Chiral Column for the Determination of 3- or 15-Acetyl Deoxynivalenol Mycotoxins from Fusarium graminearum in Wheat. Toxins. 2021; 13(9):659. https://doi.org/10.3390/toxins13090659

Chicago/Turabian Style

Wang, Lan, Zheng Yan, Haiyan Zhou, Yingying Fan, Cheng Wang, Jingbo Zhang, Yucai Liao, and Aibo Wu. 2021. "Validation of LC-MS/MS Coupled with a Chiral Column for the Determination of 3- or 15-Acetyl Deoxynivalenol Mycotoxins from Fusarium graminearum in Wheat" Toxins 13, no. 9: 659. https://doi.org/10.3390/toxins13090659

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop