Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Qin, Xing; Su, Xiaoyun; Tu, Tao; Zhang, Jie; Wang, Xiaolu; Wang, Yaru; Wang, Yuan; Bai, Yingguo; Yao, Bin; Luo, Huiying; Huang, Huoqing

doi:10.3390/toxins13060429

Open AccessArticle

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

by

Xing Qin

,

Xiaoyun Su

,

Tao Tu

,

Jie Zhang

,

Xiaolu Wang

,

Yaru Wang

,

Yuan Wang

,

Yingguo Bai

,

Bin Yao

,

Huiying Luo

^* and

Huoqing Huang

^*

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

^*

Authors to whom correspondence should be addressed.

Toxins 2021, 13(6), 429; https://doi.org/10.3390/toxins13060429

Submission received: 20 May 2021 / Revised: 9 June 2021 / Accepted: 16 June 2021 / Published: 19 June 2021

(This article belongs to the Section Mycotoxins)

Download

Browse Figures

Versions Notes

Abstract

The co-occurrence of multiple mycotoxins, including aflatoxin B₁ (AFB₁), zearalenone (ZEN) and deoxynivalenol (DON), widely exists in cereal-based animal feed and food. At present, most reported mycotoxins degrading enzymes target only a certain type of mycotoxins. Therefore, it is of great significance for mining enzymes involved in the simultaneous degradation of different types of mycotoxins. In this study, a dye-decolorizing peroxidase-encoding gene BsDyP from Bacillus subtilis SCK6 was cloned and expressed in Escherichia coli BL21/pG-Tf2. The purified recombinant BsDyP was capable of oxidizing various substrates, including lignin phenolic model compounds 2,6-dimethylphenol and guaiacol, the substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anthraquinone dye reactive blue 19 and azo dye reactive black 5, as well as Mn²⁺. In addition, BsDyP could efficiently degrade different types of mycotoxins, including AFB₁, ZEN and DON, in presence of Mn²⁺. More important, the toxicities of their corresponding enzymatic degradation products AFB₁-diol, 15-OH-ZEN and C₁₅H₁₈O₈ were significantly lower than AFB₁, ZEN and DON. In summary, these results proved that BsDyP was a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food.

Keywords: dye-decolorizing peroxidase; mycotoxin; aflatoxin B₁; zearalenone; deoxynivalenol; biotransformation

Share and Cite

MDPI and ACS Style

Qin, X.; Su, X.; Tu, T.; Zhang, J.; Wang, X.; Wang, Y.; Wang, Y.; Bai, Y.; Yao, B.; Luo, H.; et al. Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis. Toxins 2021, 13, 429. https://doi.org/10.3390/toxins13060429

AMA Style

Qin X, Su X, Tu T, Zhang J, Wang X, Wang Y, Wang Y, Bai Y, Yao B, Luo H, et al. Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis. Toxins. 2021; 13(6):429. https://doi.org/10.3390/toxins13060429

Chicago/Turabian Style

Qin, Xing, Xiaoyun Su, Tao Tu, Jie Zhang, Xiaolu Wang, Yaru Wang, Yuan Wang, Yingguo Bai, Bin Yao, Huiying Luo, and et al. 2021. "Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis" Toxins 13, no. 6: 429. https://doi.org/10.3390/toxins13060429

APA Style

Qin, X., Su, X., Tu, T., Zhang, J., Wang, X., Wang, Y., Wang, Y., Bai, Y., Yao, B., Luo, H., & Huang, H. (2021). Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis. Toxins, 13(6), 429. https://doi.org/10.3390/toxins13060429

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI