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Article

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

1
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Subang Jaya 47500, Malaysia
2
Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, Malaysia
3
Kulliyyah of Pharmacy, Kuantan Campus, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan 25200, Malaysia
4
Monash Venom Group, Department of Pharmacology, Biomedical Discovery Institute, Monash University, Clayton, VIC 3800, Australia
*
Authors to whom correspondence should be addressed.
Toxins 2021, 13(12), 859; https://doi.org/10.3390/toxins13120859
Received: 15 October 2021 / Revised: 22 November 2021 / Accepted: 27 November 2021 / Published: 2 December 2021
(This article belongs to the Special Issue Venom-Induced Tissue Damage)
Phospholipase A2 (PLA2) toxins are one of the main toxin families found in snake venom. PLA2 toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although Naja sumatrana venom contains substantial quantities of PLA2 components, there is limited information on the function and activities of PLA2 toxins from the venom. In this study, a secretory PLA2 from the venom of Malaysian N. sumatrana, subsequently named A2-EPTX-Nsm1a, was isolated, purified, and characterized. A2-EPTX-Nsm1a was purified using a mass spectrometry-guided approach and multiple chromatography steps. Based on LC-MSMS, A2-EPTX-Nsm1a was found to show high sequence similarity with PLA2 from venoms of other Naja species. The PLA2 activity of A2-EPTX-Nsm1 was inhibited by 4-BPB and EDTA. A2-EPTX-Nsm1a was significantly less cytotoxic in a neuroblastoma cell line (SH-SY5Y) compared to crude venom and did not show a concentration-dependent cytotoxic activity. To our knowledge, this is the first study that characterizes and investigates the cytotoxicity of an Asp49 PLA2 isolated from Malaysian N. sumatrana venom in a human neuroblastoma cell line. View Full-Text
Keywords: Naja sumatrana; spitting cobra; snake venom phospholipase; phospholipase A2 Naja sumatrana; spitting cobra; snake venom phospholipase; phospholipase A2
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MDPI and ACS Style

Abdullah, N.A.H.; Rusmili, M.R.A.; Zainal Abidin, S.A.; Shaikh, M.F.; Hodgson, W.C.; Othman, I. Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom. Toxins 2021, 13, 859. https://doi.org/10.3390/toxins13120859

AMA Style

Abdullah NAH, Rusmili MRA, Zainal Abidin SA, Shaikh MF, Hodgson WC, Othman I. Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom. Toxins. 2021; 13(12):859. https://doi.org/10.3390/toxins13120859

Chicago/Turabian Style

Abdullah, Nur A.H., Muhamad R.A. Rusmili, Syafiq A. Zainal Abidin, Mohd F. Shaikh, Wayne C. Hodgson, and Iekhsan Othman. 2021. "Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom" Toxins 13, no. 12: 859. https://doi.org/10.3390/toxins13120859

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