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Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom

1
Laboratory of Translational Immunology, Medicine School, FCM, University of Campinas (UNICAMP), Campinas 13083-888, Brazil
2
Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, Institute of Biosciences of Rio Claro, São Paulo State University, Rio Claro 13500, Brazil
3
Laboratory of Arthropods Molecular Biology - LBMA-IBRC-UNESP (São Paulo State University), Rio Claro 13506-900, Brazil
4
Venoms and Venomous Animal Studies Center-CEVAP, São Paulo State University (UNESP), Botucatu 18610-307, Brazil
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(6), 379; https://doi.org/10.3390/toxins12060379
Received: 25 March 2020 / Revised: 3 April 2020 / Accepted: 6 April 2020 / Published: 8 June 2020
(This article belongs to the Special Issue Application of Venom Phospholipase in the Treatment of Diseases)
Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy. View Full-Text
Keywords: Hymenoptera venom allergy; Polybia paulista; CD4+ T cells Hymenoptera venom allergy; Polybia paulista; CD4+ T cells
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Fernandes, L.G.R.; Perez-Riverol, A.; Bazon, M.L.; Abram, D.M.; Brochetto-Braga, M.R.; Zollner, R.L. Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom. Toxins 2020, 12, 379.

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