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Open AccessArticle

Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance

1
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, China
2
International Crops Research Institute of the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India
*
Author to whom correspondence should be addressed.
Toxins 2020, 12(3), 156; https://doi.org/10.3390/toxins12030156
Received: 12 February 2020 / Revised: 20 February 2020 / Accepted: 28 February 2020 / Published: 1 March 2020
(This article belongs to the Special Issue Novel Strategy to Prevent Post-Harvest Mycotoxin Contamination)
Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) are the most common aflatoxins produced by Aspergillus flavus in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to offer breeders efficient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%–18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%–31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts. View Full-Text
Keywords: aflatoxin resistance; peanut; genome-wide association study (GWAS); mini-mini core collection; restriction site associated DNA sequencing (RAD-Seq) aflatoxin resistance; peanut; genome-wide association study (GWAS); mini-mini core collection; restriction site associated DNA sequencing (RAD-Seq)
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Yu, B.; Jiang, H.; Pandey, M.K.; Huang, L.; Huai, D.; Zhou, X.; Kang, Y.; Varshney, R.K.; Sudini, H.K.; Ren, X.; Luo, H.; Liu, N.; Chen, W.; Guo, J.; Li, W.; Ding, Y.; Jiang, Y.; Lei, Y.; Liao, B. Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance. Toxins 2020, 12, 156.

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