The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin
Abstract
1. Introduction
2. Results
2.1. Detection of S. maltophilia TA Systems in Genomes
2.2. The Prevalence of TA Systems in Stenotrophomonas spp. of Clinical and Environmental Origin
2.3. Functionality of S. maltophilia TA Systems
2.4. Characterization of Stenotrophomonas spp. Isolates
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Bioinformatic Assays
5.2. The Bacteria Used in the Study and Growth Conditions
5.3. Detection of TA Systems in Stenotrophomonas
5.4. Cloning of TA System Genes
5.5. Kill-Rescue Assay
5.6. Random Amplified Polymorphic DNA (RAPD) Assay
5.7. The Evaluation of Antibiotic Resistance
5.8. Biofilm Formation Assay
5.9. Pellicle Formation Assay
5.10. Extraction of Capsular Polysaccharides
5.11. Serum Resistance
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Predicted TA Family | Protein Domains (T-A) 1 | Found in Genomes (n = 21) | Conservativity of Operon (%) 2 | Prevalence in Other Bacteria 3 | Remarks |
---|---|---|---|---|---|
vapBC | PIN-AbrB | 21 | 84 | Stenotrophomonas spp., Xantomonas spp. | |
- | COG3832- ArsR | 20 | 90 | Stenotrophomonas spp. and other genera | |
relBE | RelE-Xre | 15 | 73 | Stenotrophomonas spp. | T before A |
relBE | RelE-RHH | 10 | 92 | Stenotrophomonas spp. | |
relBE (higBA) | HigB-Xre | 8 | 78 | Stenotrophomonas spp., Pseudomonas spp. | T before A |
hicAB | HicA-HicB | 14 | 79 | Stenotrophomonas spp. | T before A |
hipBA | HipA-Xre | 8 | 70 | Stenotrophomonas spp. (T common to Burkholderia spp.) | T is 440 a.a. |
Strain/Plasmid | Description | Reference |
---|---|---|
E. coli JM107 | endA1 glnV44 thi-1 relA1 gyrA96 Δ(lac-proAB) [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(RK− mK+) λ− | [81] |
E. coli BW25113 F’ | proA+B+lacIqΔlacZ)M15 zzf::mini-Tn10 (KanR) | [82] |
pBAD30 | Expression plasmid | [83] |
pUHE 25-2(cat) | Expression plasmid | [82] |
pBAD1_gfp | gfp gene cloned into pBAD-MCS-1 vector | [84] |
Isolate | Genus/Species | Origin | Source 1 | Isolation Year |
---|---|---|---|---|
D27 | S. maltophilia/rhizophila | Environmental | Soil (Conventional wheat) | 2016 |
D32 | Stenotrophomonas sp. | Environmental | Soil (Conventional wheat) | 2016 |
D34 | S. maltophilia | Environmental | Soil (Conventional wheat) | 2016 |
D44 | S. maltophilia | Environmental | Soil (Conventional wheat) | 2016 |
D46 | S. maltophilia | Environmental | Soil (Conventional wheat) | 2016 |
D50 | S. maltophilia | Environmental | Soil (Conventional wheat) | 2016 |
D53 | S. maltophilia | Environmental | Soil (Conventional wheat) | 2016 |
D55 | Stenotrophomonas sp. | Environmental | Soil (Organic rapeseed) | 2016 |
D59 | Stenotrophomonas sp. | Environmental | Soil (Organic rapeseed) | 2016 |
D70 | S. rhizophila | Environmental | Soil (Organic rapeseed) | 2016 |
D73 | Stenotrophomonas sp. | Environmental | Soil (Organic rapeseed) | 2016 |
D125 | Stenotrophomonas sp. | Environmental | Soil (Organic maize) | 2016 |
D150 | Stenotrophomonas sp. | Environmental | Soil (Conventional maize) | 2016 |
Z53 | S. maltophilia | Environmental | Fish (Carp) | 2016 |
SM3 | S. maltophilia | Clinical | NCI | 2017 |
SM5 | S. maltophilia | Clinical | VCCH LM | 2018 |
SM6 | S. maltophilia | Clinical | VCCH LM | 2018 |
SM7 | S. maltophilia | Clinical | VCCH LM | 2018 |
SM8 | S. maltophilia | Clinical | NCI | 2019 |
SM9 | S. maltophilia | Clinical | NCI | 2019 |
SM10 | S. maltophilia | Clinical | VCCH LM | 2019 |
SM11 | S. maltophilia | Clinical | VUH SK PD | 2019 |
SM12 | S. maltophilia | Clinical | VUH SK PD | 2019 |
SM13 | S. maltophilia | Clinical | VUH SK PD | 2019 |
SM14 | S. maltophilia | Clinical | VCCH LM | 2019 |
SM15 | S.maltophilia | Clinical | KCPHC | 2019 |
SM16 | S. maltophilia | Clinical | VCCH LM | 2019 |
SM17 | S. maltophilia | Clinical | NCI | 2019 |
SM18 | S. maltophilia | Clinical | NCI | 2019 |
SM20 | S. maltophilia | Clinical | NCI | 2019 |
SM21 | S. maltophilia | Clinical | NCI | 2019 |
SM22 | S. maltophilia | Clinical | NCI | 2019 |
SM23 | S. maltophilia | Clinical | NCI | 2019 |
SM24 | S. maltophilia | Clinical | NCI | 2019 |
SM25 | S. maltophilia | Clinical | NCI | 2019 |
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Klimkaitė, L.; Armalytė, J.; Skerniškytė, J.; Sužiedėlienė, E. The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin. Toxins 2020, 12, 635. https://doi.org/10.3390/toxins12100635
Klimkaitė L, Armalytė J, Skerniškytė J, Sužiedėlienė E. The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin. Toxins. 2020; 12(10):635. https://doi.org/10.3390/toxins12100635
Chicago/Turabian StyleKlimkaitė, Laurita, Julija Armalytė, Jūratė Skerniškytė, and Edita Sužiedėlienė. 2020. "The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin" Toxins 12, no. 10: 635. https://doi.org/10.3390/toxins12100635
APA StyleKlimkaitė, L., Armalytė, J., Skerniškytė, J., & Sužiedėlienė, E. (2020). The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin. Toxins, 12(10), 635. https://doi.org/10.3390/toxins12100635