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The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes

1
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
2
College of Science, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
These three authors contribute equally to this work.
Toxins 2019, 11(7), 390; https://doi.org/10.3390/toxins11070390
Received: 15 June 2019 / Revised: 1 July 2019 / Accepted: 3 July 2019 / Published: 4 July 2019
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Abstract

Pomacea canaliculata hemocytes are the main functional cells in the immune defense system, and hemocyte destruction disrupts the immune response mechanism of P. canaliculata, resulting in abnormal growth, development, reproduction, and even death. Our previous study found that Pedunsaponin A significantly affects P. canaliculata hemocyte structure. This study further investigated the damaging effects of Pedunsaponin A on P. canaliculata hemocytes. The cell mortality rate results showed that the hemocyte mortality was significantly increased after treatment with Pedunsaponin A, and the mortality rate exhibited a significant positive correlation with treatment time and dose. The membrane potential results showed that the cell membranes of P. canaliculata hemocytes exhibited time-dependent membrane depolarization after 40 mg/L Pedunsaponin A treatment. At 36 h, the cell depolarization rate in the Pedunsaponin A treatment group was 41.43%, which was significantly greater than the control group (6.24%). The cytoskeleton results showed that Pedunsaponin A led to disordered and dispersed arrangement of microfilaments and changes in the cytoskeletal structure. The apoptosis and cell cycle results showed that Pedunsaponin A induced apoptosis and influenced the cell cycle to some extent. These results showed that the cell membrane and cytoskeleton of P. canaliculata hemocytes were damaged after treatment with Pedunsaponin A, which led to an increase in cell mortality, dysfunction, cell cycle abnormalities and apoptosis. This study provides a foundation for further identification of the site of Pedunsaponin A activity on hemocytes. View Full-Text
Keywords: Pomacea canaliculata; Pedunsaponin A; membrane potential; cytoskeleton; apoptosis; cell cycle Pomacea canaliculata; Pedunsaponin A; membrane potential; cytoskeleton; apoptosis; cell cycle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Yang, C.; Lv, T.; Wang, B.; Qiu, X.; Luo, L.; Zhang, M.; Yue, G.; Qin, G.; Xie, D.; Chen, H. The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes. Toxins 2019, 11, 390.

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