Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells
Abstract
:1. Introduction
2. Results
2.1. FB1 Suppressed the Viability of PIECs
2.2. FB1 Exerted Oxidative Stress in PIECs
2.3. FB1 Exacerbated the Permeability of PIECs
2.4. FB1 Destructed the Tight Junctions in PIECs
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Cell Line and Cell Culture
5.2. Cell Viability Assay by MTT
5.3. Detection of Oxidation Indices
5.4. Measurement of Paracellular Permeability
5.5. qPCR Assay
5.6. Western Blot
5.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequences | Genebank No. |
---|---|---|
Claudin 1 | F: 5′- GCAGCAGCTTCTTGCTTCTC-3′ | NM_001244539.1 |
R: 5′-CTGGCATTGACTGGGGTCAT-3′ | ||
Occludin | F: 5′- ATCAACAAAGGCAACTCT-3′ | XM_005672525.3 |
R: 5′-GCAGCAGCCATGTACTCT-3′ | ||
ZO-1 | F: 5′- GAGTTTGATAGTGGCGTT-3′ | XM_021098896.1 |
R: 5′- GTGGGAGGATGCTGTTGT-3′ | ||
GAPDH | F: 5′- ACAGGGTGGTGGACCTCATG-3′ | XM_021091114.1 |
R: 5′-GGGTCTGGGATGGAAACTGG-3′ |
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Yuan, Q.; Jiang, Y.; Fan, Y.; Ma, Y.; Lei, H.; Su, J. Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells. Toxins 2019, 11, 387. https://doi.org/10.3390/toxins11070387
Yuan Q, Jiang Y, Fan Y, Ma Y, Lei H, Su J. Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells. Toxins. 2019; 11(7):387. https://doi.org/10.3390/toxins11070387
Chicago/Turabian StyleYuan, Qiaoling, Yancheng Jiang, Ying Fan, Yingfeng Ma, Hongyu Lei, and Jianming Su. 2019. "Fumonisin B1 Induces Oxidative Stress and Breaks Barrier Functions in Pig Iliac Endothelium Cells" Toxins 11, no. 7: 387. https://doi.org/10.3390/toxins11070387