Next Article in Journal
Prey and Venom Efficacy of Male and Female Wandering Spider, Phoneutria boliviensis (Araneae: Ctenidae)
Previous Article in Journal
Meteorological and Nutrient Conditions Influence Microcystin Congeners in Freshwaters
Open AccessArticle

Determination of Aflatoxin B1 and B2 in Vegetable Oils Using Fe3O4/rGO Magnetic Solid Phase Extraction Coupled with High-Performance Liquid Chromatography Fluorescence with Post-Column Photochemical Derivatization

by Li Yu 1,2,3,†, Fei Ma 1,2,3,4,†, Liangxiao Zhang 1,2,4,5 and Peiwu Li 1,2,3,4,5,*
1
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
4
Laboratory of Quality and Safety Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
5
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Toxins 2019, 11(11), 621; https://doi.org/10.3390/toxins11110621
Received: 12 September 2019 / Revised: 18 October 2019 / Accepted: 24 October 2019 / Published: 26 October 2019
(This article belongs to the Section Mycotoxins)
In this study, magnetic graphene nanocomposite Fe3O4/rGO was synthesized by facile one-pot solvothermal method. The nanocomposite was successfully used as magnetic solid phase extraction (MSPE) adsorbents for the determination of aflatoxins in edible vegetable oils through the π–π stacking interactions. MSPE parameters including the amount of adsorbents, extraction and desorption time, washing conditions, and the type and volume of desorption solvent were optimized. Under optimal conditions, good linear relationships were achieved. Limits of detection of this method were as low as 0.02 µg/kg and 0.01 µg/kg for aflatoxin B1 and B2, respectively. Finally, the magnetic graphene nanocomposite was successfully applied to aflatoxin analysis in vegetable oils. The results indicated that the recoveries of the B-group aflatoxins ranged from 80.4% to 106.0%, whereas the relative standard deviations (RSDs) were less than 8.1%. Owing to the simplicity, rapidity and efficiency, Fe3O4/rGO magnetic solid phase extraction coupled with high-performance liquid chromatography fluorescence with post-column photochemical derivatization (Fe3O4/rGO MSPE-HPLC-PCD-FLD) is a promising analytical method for routine and accurate determination of aflatoxins in lipid matrices. View Full-Text
Keywords: aflatoxin; magnetic solid phase extraction; graphene; high-performance liquid chromatography fluorescence; vegetable oil aflatoxin; magnetic solid phase extraction; graphene; high-performance liquid chromatography fluorescence; vegetable oil
Show Figures

Figure 1

MDPI and ACS Style

Yu, L.; Ma, F.; Zhang, L.; Li, P. Determination of Aflatoxin B1 and B2 in Vegetable Oils Using Fe3O4/rGO Magnetic Solid Phase Extraction Coupled with High-Performance Liquid Chromatography Fluorescence with Post-Column Photochemical Derivatization. Toxins 2019, 11, 621.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop