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Open AccessArticle

Sa12b Peptide from Solitary Wasp Inhibits ASIC Currents in Rat Dorsal Root Ganglion Neurons

1
Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico
2
Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan
3
Laboratório de Genética, Instituto Butantan, São Paulo 05503-900, Brazil
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(10), 585; https://doi.org/10.3390/toxins11100585
Received: 30 July 2019 / Revised: 23 September 2019 / Accepted: 4 October 2019 / Published: 10 October 2019
(This article belongs to the Special Issue Arthropod Venom Components and Their Potential Usage)
In this work, we evaluate the effect of two peptides Sa12b (EDVDHVFLRF) and Sh5b (DVDHVFLRF-NH2) on Acid-Sensing Ion Channels (ASIC). These peptides were purified from the venom of solitary wasps Sphex argentatus argentatus and Isodontia harmandi, respectively. Voltage clamp recordings of ASIC currents were performed in whole cell configuration in primary culture of dorsal root ganglion (DRG) neurons from (P7-P10) CII Long-Evans rats. The peptides were applied by preincubation for 25 s (20 s in pH 7.4 solution and 5 s in pH 6.1 solution) or by co-application (5 s in pH 6.1 solution). Sa12b inhibits ASIC current with an IC50 of 81 nM, in a concentration-dependent manner when preincubation application was used. While Sh5b did not show consistent results having both excitatory and inhibitory effects on the maximum ASIC currents, its complex effect suggests that it presents a selective action on some ASIC subunits. Despite the similarity in their sequences, the action of these peptides differs significantly. Sa12b is the first discovered wasp peptide with a significant ASIC inhibitory effect. View Full-Text
Keywords: venom peptides; FMRF-amide; insect neurotoxin; protons; pH regulation; acid-sensing ion channels; acid-gated currents venom peptides; FMRF-amide; insect neurotoxin; protons; pH regulation; acid-sensing ion channels; acid-gated currents
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MDPI and ACS Style

Hernández, C.; Konno, K.; Salceda, E.; Vega, R.; Zaharenko, A.J.; Soto, E. Sa12b Peptide from Solitary Wasp Inhibits ASIC Currents in Rat Dorsal Root Ganglion Neurons. Toxins 2019, 11, 585.

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